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Cytochrome C 抗体

CYCS 适用: 人, 大鼠, 小鼠, 马, 犬, Pigeon, Drosophila melanogaster, Frog WB, IHC, IF, FACS 宿主: 小鼠 Monoclonal unconjugated
产品编号 ABIN6297608
发货至: 中国
  • 抗原 See all Cytochrome C (CYCS) 抗体
    Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
    适用
    • 142
    • 110
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    人, 大鼠, 小鼠, 马, 犬, Pigeon, Drosophila melanogaster, Frog
    宿主
    • 88
    • 67
    • 5
    • 1
    小鼠
    克隆类型
    • 81
    • 79
    • 1
    单克隆
    标记
    • 94
    • 11
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    This Cytochrome C antibody is un-conjugated
    应用范围
    • 124
    • 48
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    • 32
    • 28
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    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
    原理
    Mouse anti-Human/Mouse/Rat/Horse/Dog/Pigeon/Frog/Drosophila Cytochrome C Antibody [Sodium Azide Free]
    特异性
    Cytoplasmic
    纯化方法
    Recombinant protein was used as the immunogen for the Cytochrome C antibody.
    免疫原
    Recombinant protein was used as the immunogen for the Cytochrome C antibody.
    Top Product
    Discover our top product CYCS Primary Antibody
  • 应用备注
    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
    Immunofluorescence: 0.5-1 μg/mL
    Western blot: 0.5-1 μg/mL
    Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
    Prediluted format: incubate for 30 min at RT (2)
    限制
    仅限研究用
  • 缓冲液
    In 1X PBS, BSA free, sodium azide free
    储存液
    Azide free
    储存条件
    4 °C,-20 °C
    储存方法
    2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
  • 抗原
    Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
    别名
    Cytochrome c (CYCS 产品)
    背景

    Target Description: Cytochrome C is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.

    Gene Symbol: CYCS///CYC

    基因ID
    54205
    UniProt
    P99999
    途径
    Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
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