MGMT 抗体 (N-Term)
Quick Overview for MGMT 抗体 (N-Term) (ABIN6263244)
抗原
See all MGMT 抗体适用
宿主
克隆类型
标记
应用范围
-
-
抗原表位
- N-Term
-
特异性
- MGMT Antibody detects endogenous levels of total MGMT.
-
纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
-
免疫原
- A synthesized peptide derived from human MGMT, corresponding to a region within N-terminal amino acids.
-
亚型
- IgG
-
-
-
-
应用备注
- WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
-
有效期
- 12 months
-
-
- MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
-
别名
- MGMT
-
背景
-
Description: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Gene: MGMT
-
分子量
- 22 kDa
-
基因ID
- 4255
-
UniProt
- P16455
-
途径
- DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
抗原
-