LEF1 抗体 (Internal Region)
Quick Overview for LEF1 抗体 (Internal Region) (ABIN6262946)
抗原
See all LEF1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- LEF1 Antibody detects endogenous levels of total LEF1.
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预测反应
- Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human LEF1, corresponding to a region within the internal amino acids.
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亚型
- IgG
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anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 100-399) antibody
LEF1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 331-399) antibodyLEF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 1-399) antibodyLEF1 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 3H5 unconjugated
anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (Internal Region) antibodyVerified LEF1 适用: 人 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 33-138) antibodyLEF1 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 1A4B11 unconjugated
anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 14-123) antibodyLEF1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 5A3 unconjugated
anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 75-216) antibodyLEF1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 33-138) antibodyLEF1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1A8 unconjugated
anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 10-37), (N-Term) antibodyLEF1 适用: 人 WB, IF 宿主: 兔 Polyclonal RB31989 unconjugated
anti-Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 51-100) antibodyLEF1 适用: 人, 犬, 豚鼠, Cow, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:1000-3000, IHC 1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))
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别名
- LEF1
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背景
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Description: Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
Gene: LEF1
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分子量
- 55 kDa
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基因ID
- 51176
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UniProt
- Q9UJU2
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途径
- WNT signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding
抗原
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