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APEX1 抗体 (N-Term)

Name: APEX1 抗体 (N-Term)
SKU: ABIN6259977
Availability: OutOfStock

This anti-APEX1 antibody is a 兔多克隆 antibody detecting APEX1 in WB, ELISA, IHC, IF 和 ICC. Suitable for 人, 小鼠和大鼠.

Western blot analysis of extracts of Hela, using APEX1 antibody. (APEX1 抗体 (N-Term))

ABIN6276522 staining HEPG2 cells by ICC/IF. (APEX1 抗体 (N-Term))

IF/ICC

ABIN6276522 at 1/100 staining Mouse spleen tissue by IHC-P. (APEX1 抗体 (N-Term))

IHC

IF/ICC

4 images

产品编号 ABIN6259977

发货至: 中国

Quick Overview for APEX1 抗体 (N-Term) (ABIN6259977)

抗原

See all APEX1 抗体

APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This APEX1 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Discover our top product APEX1 Primary Antibody

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N-Term

特异性

APEX1 Antibody detects endogenous levels of total APEX1.

预测反应

Pig,Bovine,Horse,Sheep,Rabbit,Dog

纯化方法

The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

免疫原

A synthesized peptide derived from human APEX1, corresponding to a region within N-terminal amino acids.

亚型

IgG
anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 1-318) antibody
APEX1 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated

anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 1-242) antibody
APEX1 适用: 人, 犬, 猴 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 1H9 unconjugated

anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 219-318) antibody
APEX1 适用: 人, 大鼠, 猴 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 7A2G7 unconjugated

anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 1-318) antibody
APEX1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 1-318) antibody
APEX1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 2-318) antibody
APEX1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) antibody
APEX1 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 1518CT337-123-86-269-232 unconjugated

anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (acLys6) antibody
APEX1 适用: 人 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) (AA 1-318) antibody
APEX1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Apurinic/Apyrimidinic Endonuclease 1 (APEX1) antibody
APEX1 适用: 人, 小鼠, 大鼠, 兔 WB, IHC, IF, ICC, ChIP, IP, Neut 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:400, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
抗原

APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

别名

APEX1

背景

Description: Multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Initiates repair of AP sites in DNA by catalyzing hydrolytic incision of the phosphodiester backbone immediately adjacent to the damage, generating a single-strand break with 5'-deoxyribose phosphate and 3'-hydroxyl ends. Does also incise at AP sites in the DNA strand of DNA/RNA hybrids, single-stranded DNA regions of R-loop structures, and single-stranded RNA molecules. Has a 3'-5' exoribonuclease activity on mismatched deoxyribonucleotides at the 3' termini of nicked or gapped DNA molecules during short-patch BER. Possesses a DNA 3' phosphodiesterase activity capable of removing lesions (such as phosphoglycolate) blocking the 3' side of DNA strand breaks. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5'-terminal deoxyribose 5'-phosphate (dRp) excision activity of POLB. Plays a role in the protection from granzymes-mediated cellular repair leading to cell death. Also involved in the DNA cleavage step of class switch recombination (CSR). On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. Involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). Together with HNRNPL or the dimer XRCC5/XRCC6, associates with nCaRE, acting as an activator of transcriptional repression. Stimulates the YBX1-mediated MDR1 promoter activity, when acetylated at Lys-6 and Lys-7, leading to drug resistance. Acts also as an endoribonuclease involved in the control of single-stranded RNA metabolism. Plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, plays a role in the rRNA quality control process during cell cycle progression. Associates, together with YBX1, on the MDR1 promoter. Together with NPM1, associates with rRNA. Binds DNA and RNA.
Gene: APEX1

分子量

36kDa

基因ID

328

UniProt

P27695

途径

DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus