AP2M1 抗体 (Internal Region)
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- 抗原 See all AP2M1 抗体
- AP2M1 (Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1))
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抗原表位
- Internal Region
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This AP2M1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 特异性
- AP2M1 Antibody detects endogenous levels of total AP2M1.
- 预测反应
- Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
- 纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- 免疫原
- A synthesized peptide derived from human AP2M1, corresponding to a region within the internal amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product AP2M1 Primary Antibody
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anti-Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1) (AA 97-383) antibody
AP2M1 适用: 人, 大鼠, 犬 WB, IHC, FACS, IHC (p) 宿主: 小鼠 Monoclonal 2F11 unconjugated
anti-Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1) (AA 97-383) antibodyAP2M1 适用: 人, 小鼠 WB, FACS 宿主: 小鼠 Monoclonal 2G2 unconjugated
anti-Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1) (AA 298-435) antibodyAP2M1 适用: 人, 猴 WB, ELISA, IHC, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 4F1B2 unconjugated
anti-Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1) (AA 136-435) antibodyAP2M1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1) (AA 1-435) antibodyAP2M1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1) (AA 134-433) antibodyAP2M1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1) antibodyAP2M1 适用: 人 WB, IF 宿主: 兔 Monoclonal unconjugated
anti-Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1) (Center) antibodyAP2M1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1) (N-Term) antibodyAP2M1 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1) (AA 60-278) antibodyAP2M1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
- 有效期
- 12 months
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- 抗原
- AP2M1 (Adaptor-Related Protein Complex 2, mu 1 Subunit (AP2M1))
- 别名
- AP2M1 (AP2M1 产品)
- 别名
- AP50 antibody, CLAPM1 antibody, mu2 antibody, Ap50 antibody, Clapm1 antibody, cb34 antibody, Ap2m1 antibody, dpy-23 antibody, sb:cb34 antibody, zgc:55711 antibody, zgc:85653 antibody, wu:fa97a10 antibody, ap2m1-a antibody, ap2m1 antibody, zgc:56643 antibody, AP2M1 antibody, adaptor related protein complex 2 mu 1 subunit antibody, adaptor-related protein complex 2, mu 1 subunit antibody, adaptor-related protein complex 2, mu 1 subunit, a antibody, adaptor related protein complex 2 mu 1 subunit L homeolog antibody, adaptor-related protein complex 2, mu 1 subunit, b antibody, AP-2 complex subunit mu-1 antibody, clathrin coat assembly protein AP50 antibody, clathrin coat assembly protein ap50 antibody, AP2M1 antibody, Ap2m1 antibody, ap2m1a antibody, ap2m1.L antibody, ap2m1b antibody, ap2m1 antibody, cgd8_950 antibody, PY06523 antibody, PVX_118455 antibody, PVX_123590 antibody, CpipJ_CPIJ003697 antibody
- 背景
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Description: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins, it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in membrane-associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling (By similarity).
Gene: AP2M1
- 分子量
- 49kDa
- 基因ID
- 1173
- UniProt
- Q96CW1
- 途径
- EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation, SARS-CoV-2 Protein Interactome
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