TDG 抗体 (Internal Region)
Quick Overview for TDG 抗体 (Internal Region) (ABIN6258811)
抗原
See all TDG 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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特异性
- TDG Antibody detects endogenous levels of total TDG.
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预测反应
- Pig,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human TDG, corresponding to a region within the internal amino acids.
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亚型
- IgG
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anti-Thymine-DNA Glycosylase (TDG) (AA 141-410) antibody
TDG 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Thymine-DNA Glycosylase (TDG) (AA 1-410) antibodyTDG 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Thymine-DNA Glycosylase (TDG) antibodyTDG 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Thymine-DNA Glycosylase (TDG) (AA 1-410) antibodyTDG 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Thymine-DNA Glycosylase (TDG) antibodyTDG 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Thymine-DNA Glycosylase (TDG) (AA 281-310), (C-Term) antibodyTDG 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB19894 unconjugated
anti-Thymine-DNA Glycosylase (TDG) (AA 81-110), (N-Term) antibodyTDG 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB19893 unconjugated
anti-Thymine-DNA Glycosylase (TDG) (Center) antibodyTDG 适用: 人, 小鼠, 大鼠, Cow WB, IF, IC 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- TDG (Thymine-DNA Glycosylase (TDG))
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别名
- TDG
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背景
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Description: DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.
Gene: TDG
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分子量
- 46 kDa
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基因ID
- 6996
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UniProt
- Q13569
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途径
- DNA Damage Repair, Chromatin Binding
抗原
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