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GRLF1 抗体 (pTyr1105)

Name: GRLF1 抗体 (pTyr1105)
SKU: ABIN6256617
Availability: OutOfStock

GRLF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated

Western blot analysis of GRF-1 phosphorylation expression in EGF treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (GRLF1 抗体 (pTyr1105))

IF/ICC

Western blot analysis of Phospho-GRF-1 (Tyr1105) expression in various lysates (GRLF1 抗体 (pTyr1105))

ABIN6267693 at 1/100 staining Human lung tissue by IHC-P. (GRLF1 抗体 (pTyr1105))

IHC

4 images

产品编号 ABIN6256617

发货至: 中国

Datasheet as PDF

抗原 See all GRLF1 抗体

GRLF1 (Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1))
抗原表位
13

4

2

2

1

1

1

1

1

1

1

1

1
pTyr1105
适用
25

20

20

4

3

3

3

3

2

1

1

1
人, 小鼠, 大鼠
宿主
25

1
兔
Compatible Secondaries
克隆类型
25

1
多克隆
标记
19

1

1

1

1

1

1

1
This GRLF1 antibody is un-conjugated
应用范围
15

12

4

3

1

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

特异性

Phospho-GRF-1 (Tyr1105) Antibody detects endogenous levels of GRF-1 only when phosphorylated at Tyrosine 1105.

预测反应

Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

纯化方法

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

免疫原

A synthesized peptide derived from human GRF-1 around the phosphorylation site of Tyr1105.

亚型

IgG
anti-Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1) (pTyr1087) antibody
GRLF1 适用: 小鼠, 大鼠 ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1) (pTyr1087) antibody
GRLF1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1) (pTyr1105) antibody
GRLF1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1) (pTyr1105) antibody
GRLF1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1) (AA 1090-1135), (pTyr1105) antibody
GRLF1 适用: 人 WB 宿主: 兔 Polyclonal RB46920 unconjugated

anti-Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1) (pTyr1087) antibody
GRLF1 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1) (pTyr1105) antibody
GRLF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1) (AA 1-36) antibody
GRLF1 适用: 人 ELISA 宿主: 小鼠 Monoclonal 4D4-F8 unconjugated

anti-Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1) (AA 44-93) antibody
GRLF1 适用: 人, 小鼠, 大鼠, 犬, Pig, Cow, 豚鼠, 马, 兔, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated

anti-Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1) (AA 1071-1120), (pTyr1105) antibody
GRLF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
抗原

GRLF1 (Glucocorticoid Receptor DNA Binding Factor 1 (GRLF1))

别名

ARHGAP35 (GRLF1 产品)

别名

GRF-1 antibody, GRLF1 antibody, P190-A antibody, P190A antibody, p190ARhoGAP antibody, p190RhoGAP antibody, 6430596G11Rik antibody, AI841135 antibody, Arhgap35 antibody, mKIAA1722 antibody, p190A antibody, Grlf1 antibody, p190-A antibody, arhgap35 antibody, grf-1 antibody, grlf1 antibody, p190-a antibody, p190a antibody, p190arhogap antibody, p190rhogap antibody, Rho GTPase activating protein 35 antibody, Rho GTPase activating protein 35 L homeolog antibody, ARHGAP35 antibody, Arhgap35 antibody, arhgap35.L antibody

背景

Description: Rho GTPase-activating protein (GAP) (PubMed:19673492, PubMed:28894085). Binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity (PubMed:19673492). This binding is inhibited by phosphorylation by PRKCA (PubMed:19673492). Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis (By similarity). Transduces signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP) (By similarity). Transduces SRC-dependent signals from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation (By similarity). Modulates Rho GTPase-dependent F-actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation (By similarity). During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth (By similarity). Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3', this function is however unclear and would need additional experimental evidences (PubMed:1894621).
Gene: ARHGAP35

分子量

155kDa

基因ID

2909

UniProt

Q9NRY4

途径

Tube Formation