TP53BP1 抗体 (pThr543)
Quick Overview for TP53BP1 抗体 (pThr543) (ABIN6256578)
抗原
See all TP53BP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr543
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特异性
- Phospho-53BP1 (Thr543) Antibody detects endogenous levels of 53BP1 only when phosphorylated at Thr543
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交叉反应
- 人, 猴
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纯化方法
- The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
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免疫原
- A synthesized peptide derived from human Phospho-53BP1 (Thr543)
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亚型
- IgG
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anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) (AA 574-773) antibody
TP53BP1 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 6B3E10 unconjugated
anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) (N-Term) antibodyKO Validated TP53BP1 适用: 人 WB, IF, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) (AA 1955-1972), (C-Term) antibodyTP53BP1 适用: 人, 小鼠, 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) (pSer25), (pSer29) antibodyTP53BP1 适用: 人, 小鼠, 大鼠 ELISA, ICC, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) (N-Term) antibodyTP53BP1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) (AA 1763-1977) antibodyTP53BP1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) (N-Term) antibodyTP53BP1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) (AA 1724-1964) antibodyTP53BP1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) (AA 1758-1972) antibodyTP53BP1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 Binding Protein 1 (TP53BP1) (pSer1778) antibodyTP53BP1 适用: 人 WB 宿主: 兔 Monoclonal D4 unconjugated
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应用备注
- WB 1:1000-3000, IF/ICC 1:100-1:500
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C.Stable for 12 months from date of receipt
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有效期
- 12 months
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- TP53BP1 (Tumor Protein P53 Binding Protein 1 (TP53BP1))
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别名
- 53BP1
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背景
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Description: Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis (PubMed:12364621, PubMed:22553214, PubMed:23333306, PubMed:17190600, PubMed:21144835, PubMed:28241136). Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1 (PubMed:22553214, PubMed:23727112, PubMed:23333306). In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites (PubMed:28241136). Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites (PubMed:23760478, PubMed:28241136, PubMed:17190600). Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:23345425). Participates to the repair and the orientation of the broken DNA ends during CSR (By similarity). In contrast, it is not required for classic NHEJ and V(D)J recombination (By similarity). Promotes NHEJ of dysfunctional telomeres via interaction with PAXIP1 (PubMed:23727112).
Gene: TP53BP1
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分子量
- 220kDa
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基因ID
- 7158
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UniProt
- Q12888
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途径
- DNA Damage Repair
抗原
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