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E2F1 抗体 (pThr433)

This anti-E2F1 antibody is a 兔 多克隆 antibody detecting E2F1 in WB, ELISA, IF 和 ICC. Suitable for 人 和 小鼠.
产品编号 ABIN6256563
发货至: 中国

Quick Overview for E2F1 抗体 (pThr433) (ABIN6256563)

抗原

See all E2F1 抗体
E2F1 (E2F Transcription Factor 1 (E2F1))

适用

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人, 小鼠

宿主

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克隆类型

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多克隆

标记

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This E2F1 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
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    pThr433

    特异性

    Phospho-E2F1 (Thr433) Antibody detects endogenous levels of E2F1 only when phosphorylated at Threonine 433.

    预测反应

    Pig,Bovine,Horse,Rabbit,Chicken

    纯化方法

    The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

    免疫原

    A synthesized peptide derived from human E2F1 around the phosphorylation site of Thr433.

    亚型

    IgG
  • 应用备注

    WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store at -20 °C. Stable for 12 months from date of receipt.

    有效期

    12 months
  • 抗原

    E2F1 (E2F Transcription Factor 1 (E2F1))

    别名

    E2F1

    背景

    Description: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812). Positively regulates transcription of RRP1B (PubMed:20040599).

    Gene: E2F1

    分子量

    47kDa

    基因ID

    1869

    UniProt

    Q01094

    途径

    p53 Pathway, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy
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