我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

PAK1 抗体 (pThr212)

Name: PAK1 抗体 (pThr212)
SKU: ABIN6256507
Availability: OutOfStock

This anti-PAK1 antibody is a 兔多克隆 antibody detecting PAK1 in WB, IHC, ELISA, IF 和 ICC. Suitable for 人, 大鼠和小鼠.

Western blot analysis of PAK1 phosphorylation expression in etoposide treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (PAK1 抗体 (pThr212))

ABIN6267633 at 1/100 staining human liver carcinoma tissue sections by IHC-P. (PAK1 抗体 (pThr212))

IHC

IF/ICC

Western blot analysis of PAK1 phosphorylation expression in mouse brain tissue lysates,The lane on the right is treated with the antigen-specific peptide. (PAK1 抗体 (pThr212))

Western blot analysis of extracts from mouse brain, using Phospho-PAK1 (Thr212) Antibody. (PAK1 抗体 (pThr212))

5 images

产品编号 ABIN6256507

发货至: 中国

Quick Overview for PAK1 抗体 (pThr212) (ABIN6256507)

抗原

See all PAK1 抗体

PAK1 (P21-Activated Kinase 1 (PAK1))

适用

人, 大鼠, 小鼠

宿主

兔

克隆类型

多克隆

标记

This PAK1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Discover our top product PAK1 Primary Antibody

抗原表位
27

22

15

13

11

9

8

8

7

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pThr212

特异性

Phospho-PAK1 (Thr212) Antibody detects endogenous levels of PAK1 only when phosphorylated at Threonine 212.

预测反应

Pig,Bovine,Horse,Sheep,Rabbit,Dog

纯化方法

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

免疫原

A synthesized peptide derived from human PAK1 around the phosphorylation site of Thr212.

亚型

IgG
anti-P21-Activated Kinase 1 (PAK1) (AA 191-280) antibody
PAK1 适用: 人 WB, ELISA, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 4D1 unconjugated

anti-P21-Activated Kinase 1 (PAK1) (Center) antibody
PAK1 适用: 人, 大鼠, 小鼠 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-P21-Activated Kinase 1 (PAK1) (AA 252-501) antibody
PAK1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-P21-Activated Kinase 1 (PAK1) (AA 1-240) antibody
PAK1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-P21-Activated Kinase 1 (PAK1) (AA 401-430) antibody
PAK1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-P21-Activated Kinase 1 (PAK1) (Ser204) antibody
PAK1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-P21-Activated Kinase 1 (PAK1) (AA 245-490) antibody
PAK1 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-P21-Activated Kinase 1 (PAK1) (N-Term) antibody
PAK1 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-P21-Activated Kinase 1 (PAK1) (pThr423) antibody
PAK1 适用: 人 WB, IF 宿主: 兔 Polyclonal RB11381 unconjugated

anti-P21-Activated Kinase 1 (PAK1) (Ser204) antibody
PAK1 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
抗原

PAK1 (P21-Activated Kinase 1 (PAK1))

别名

PAK1

背景

Description: Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases. Involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes. Phosphorylates the tubulin chaperone TBCB and thereby plays a role in the regulation of microtubule biogenesis and organization of the tubulin cytoskeleton. Plays a role in the regulation of insulin secretion in response to elevated glucose levels. Part of a ternary complex that contains PAK1, DVL1 and MUSK that is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Phosphorylates MYL9/MLC2. Phosphorylates RAF1 at 'Ser-338' and 'Ser-339' resulting in: activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2. Phosphorylates SNAI1 at 'Ser-246' promoting its transcriptional repressor activity by increasing its accumulation in the nucleus. In podocytes, promotes NR3C2 nuclear localization. Required for atypical chemokine receptor ACKR2-induced phosphorylation of LIMK1 and cofilin (CFL1) and for the up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3, maybe through CFL1 phosphorylation and inactivation. Plays a role in RUFY3-mediated facilitating gastric cancer cells migration and invasion (PubMed:25766321).
Gene: PAK1

分子量

65kDa

基因ID

5058

UniProt

Q13153

途径

MAPK Pathway, RTK signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis