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LKB1 抗体 (pThr189)

Name: LKB1 抗体 (pThr189)
SKU: ABIN6256494
Availability: OutOfStock

This anti-LKB1 antibody is a 兔多克隆 antibody detecting LKB1 in WB, ELISA, IF 和 ICC. Suitable for 人和小鼠.

Western blot analysis of LKB1 phosphorylation expression in PMA treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (LKB1 抗体 (pThr189))

Western blot analysis of Phospho-LKB1 (Thr189) expression in various lysates (LKB1 抗体 (pThr189))

IF/ICC

3 images

产品编号 ABIN6256494

发货至: 中国

Datasheet as PDF

Quick Overview for LKB1 抗体 (pThr189) (ABIN6256494)

抗原

See all LKB1 (STK11) 抗体

LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

适用

人, 小鼠

宿主

兔

克隆类型

多克隆

标记

This LKB1 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Discover our top product STK11 Primary Antibody

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pThr189

特异性

Phospho-LKB1 (Thr189) Antibody detects endogenous levels of LKB1 only when phosphorylated at Threonine 189.

预测反应

Pig,Bovine,Horse,Sheep,Dog,Chicken,Xenopus

纯化方法

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

免疫原

A synthesized peptide derived from human LKB1 around the phosphorylation site of Thr189.

亚型

IgG
anti-serine/threonine Kinase 11 (STK11) (AA 134-433) antibody
STK11 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-serine/threonine Kinase 11 (STK11) (AA 14-44), (N-Term) antibody
STK11 适用: 人 WB, IF, IHC (p), FACS 宿主: 兔 Polyclonal RB3547 unconjugated

anti-serine/threonine Kinase 11 (STK11) (AA 14-44) antibody
STK11 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-serine/threonine Kinase 11 (STK11) (pSer428) antibody
STK11 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-serine/threonine Kinase 11 (STK11) (AA 19-48) antibody
STK11 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-serine/threonine Kinase 11 (STK11) (AA 1-433) antibody
STK11 适用: 人 WB, IF, PLA 宿主: 兔 Polyclonal unconjugated

anti-serine/threonine Kinase 11 (STK11) (Internal Region) antibody
STK11 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-serine/threonine Kinase 11 (STK11) (N-Term) antibody
STK11 适用: 人, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-serine/threonine Kinase 11 (STK11) antibody
STK11 适用: 人, 小鼠, 猴 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 4H12 unconjugated

anti-serine/threonine Kinase 11 (STK11) (AA 393-420), (C-Term) antibody
STK11 适用: 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB37144 unconjugated

应用备注

WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
抗原

LKB1 (STK11) (serine/threonine Kinase 11 (STK11))

别名

STK11

背景

Description: Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, thus promoting their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA, PTEN and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including: inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neuron polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation. Able to phosphorylate p53/TP53, the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1. Also acts as a mediator of p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to the mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways. In vein endothelial cells, inhibits PI3K/Akt signaling activity and thus induces apoptosis in response to the oxidant peroxynitrite (in vitro). Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with NUAK1, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair (PubMed:25329316).
Gene: STK11

分子量

65kDa

基因ID

6794

UniProt

Q15831

途径

AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect