TAG-72 抗体
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- 抗原 See all TAG-72 抗体
- TAG-72 (Temporarily Assigned Gene Name Family Member (TAG-72))
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适用
- 人, 大鼠, Cow, 犬
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This TAG-72 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Purified TAG-72 protein was used as the immunogen for this TAG-72 antibody.
- 克隆位点
- CC49
- 亚型
- IgG1
- Top Product
- Discover our top product TAG-72 Primary Antibody
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anti-Temporarily Assigned Gene Name Family Member (TAG-72) antibody
TAG-72 适用: 人 IHC (p), WB 宿主: 小鼠 Monoclonal SPM148 unconjugated
anti-Temporarily Assigned Gene Name Family Member (TAG-72) antibodyTAG-72 适用: 人, 大鼠, Cow, 犬 FACS, IF, IHC (p), WB 宿主: 小鼠 Monoclonal CC49 unconjugated
anti-Temporarily Assigned Gene Name Family Member (TAG-72) antibodyTAG-72 适用: 人 FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal TAGP-4 unconjugated
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- 应用备注
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Western blot: 1-2 μg/mL
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)
The optimal dilution of the TAG-72 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.2 mg/mL
- 缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- 抗原
- TAG-72 (Temporarily Assigned Gene Name Family Member (TAG-72))
- 别名
- TAG-72 (TAG-72 产品)
- 别名
- mRNA cap guanine-N7 methyltransferase antibody, tag-72 antibody
- 背景
- Recognizes an oncofetal antigen of 220 kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This mAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80 % sensitivity and 93 % specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.
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