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pan Keratin 抗体

panKRT 适用: 人, 大鼠, 小鼠, Cow, Frog, 豚鼠, Pig, 山羊, Marmoset FACS, IF, WB, IHC 宿主: 小鼠 Monoclonal SPM583 unconjugated
产品编号 ABIN6252865
发货至: 中国
  • 抗原 See all pan Keratin (panKRT) 抗体
    pan Keratin (panKRT)
    适用
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    人, 大鼠, 小鼠, Cow, Frog, 豚鼠, Pig, 山羊, Marmoset
    宿主
    • 53
    • 32
    • 1
    小鼠
    克隆类型
    • 56
    • 30
    单克隆
    标记
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    This pan Keratin antibody is un-conjugated
    应用范围
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    Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC)
    纯化方法
    Protein G affinity chromatography
    免疫原
    A keratin-enriched preparation from cultured human A431 cells was used as the immunogen for this anti-Multi-Cytokeratin antibody.
    克隆位点
    SPM583
    亚型
    IgG1
    Top Product
    Discover our top product panKRT Primary Antibody
  • 应用备注
    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 0.5-1 μg/mL

    Western blot: 0.5-1 μg/mL

    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

    Prediluted format: incubate for 30 min at RT (2)

    The optimal dilution of the anti-Multi-Cytokeratin antibody for each application should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.2 mg/mL
    缓冲液
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原
    pan Keratin (panKRT)
    别名
    Multi-Cytokeratin (panKRT 产品)
    别名
    xk70a antibody, keratin antibody, keratin S homeolog antibody, LOC431321 antibody, krt.S antibody
    背景
    This mAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
    基因ID
    3851, 3852, 3853
    UniProt
    P19013, P13647, P02538, P04259, P48668, P05787, P13645, P13646, P05783
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