Neurofilament 抗体
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北京 101111
Quick Overview for Neurofilament 抗体 (ABIN6252790)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G purified Neurofilament antibody
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免疫原
- Human recombinant Neurofilament protein was used as the immunogen. (1)
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亚型
- IgG1
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anti-Neurofilament antibody
适用: 人, 大鼠 FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal NR-4 unconjugated
anti-Neurofilament antibodyCarrier-free 适用: 人, 大鼠 FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal NR-4 unconjugated
anti-Neurofilament antibody适用: 人, 大鼠, 小鼠 FACS, IHC (p) 宿主: 小鼠 Monoclonal RT-97-NR-4 unconjugated
anti-Neurofilament antibodyCarrier-free 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal SPM563 unconjugated
anti-Neurofilament antibodyCarrier-free 适用: 人, 大鼠, 小鼠 FACS, IHC (p) 宿主: 小鼠 Monoclonal RT-97-NR-4 unconjugated
anti-Neurofilament antibodyCarrier-free 适用: 人, 大鼠, 小鼠 FACS, WB, IF, IHC (p) 宿主: 小鼠 Monoclonal NF421 unconjugated
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应用备注
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Flow Cytometry: 0.5-1 μg/million cells
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (2)
Prediluted format : incubate for 30 min at RT (3)
The usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the Neurofilament antibody to be titered for optimal performance.1. The NF421 antibody is specific to the heavy subunit.
2. FFPE tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Neurofilament
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背景
- Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). The fourth neurofilament subunit is alpha internexin in the central nervous system and peripherin in the peripheral nervous system.
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基因ID
- 4744
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UniProt
- P12036
抗原
-
