CD68 抗体
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- 抗原 See all CD68 抗体
- CD68 (CD68 Molecule (CD68))
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适用
- 人, 猴, Cat, 兔
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD68 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
- 特异性
- Does not react with Pig, Dog, Chicken
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- A human alveolar macrophage subcellular fraction was used as the immunogen for this anti-CD68 antibody.
- 克隆位点
- SPM130
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product CD68 Primary Antibody
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- 应用备注
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
The optimal dilution of the anti-CD68 antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.2 mg/mL
- 缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- 抗原
- CD68 (CD68 Molecule (CD68))
- 别名
- CD68 (CD68 产品)
- 别名
- Lamp4 antibody, Scard1 antibody, gp110 antibody, GP110 antibody, LAMP4 antibody, SCARD1 antibody, CD68 molecule antibody, CD68 antigen antibody, Cd68 molecule antibody, CD68 antibody, Cd68 antibody
- 背景
- This antibody recognizes a glycoprotein of 110 kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.
- 基因ID
- 968
- UniProt
- P34810
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