Villin 1 抗体 (AA 179-311)
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- 抗原 See all Villin 1 (VIL1) 抗体
- Villin 1 (VIL1)
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抗原表位
- AA 179-311
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Villin 1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Amino acids 179-311 from the human protein were used as the immunogen for this Villin antibody.
- 克隆位点
- VIL1-1325
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product VIL1 Primary Antibody
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anti-Villin 1 (VIL1) (AA 1-320) antibody
VIL1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Villin 1 (VIL1) (N-Term) antibodyVIL1 适用: 人 WB, IF, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Villin 1 (VIL1) (AA 1-209) antibodyVIL1 适用: 人 WB, IHC, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 3E5G11 unconjugated
anti-Villin 1 (VIL1) (AA 1-209) antibodyVIL1 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 5E3B2 unconjugated
anti-Villin 1 (VIL1) (AA 180-207), (N-Term) antibodyVIL1 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB17824 unconjugated
anti-Villin 1 (VIL1) (AA 1-421) antibodyVIL1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Villin 1 (VIL1) (AA 179-311) antibodyVIL1 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal VIL1-1314 unconjugated
anti-Villin 1 (VIL1) (AA 1-421) antibodyVIL1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2F10 unconjugated
anti-Villin 1 (VIL1) (AA 179-311) antibodyVIL1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal rVIL1-1325 unconjugated Recombinant Antibody
anti-Villin 1 (VIL1) (AA 600-700) antibodyVIL1 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-459R unconjugated Recombinant Antibody
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- 应用备注
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Flow Cytometry: 0.5-1 μg/10^6 cells in 0.1ml
IF: 1-2 μg/mL
Western blot: 1-2 μg/mL
IHC (FFPE): 0.25-0.5 μg/mL for 30 min at RT
The stated application concentrations are suggested starting points. Titration of the Villin antibody may be required due to differences in protocols and secondary/substrate sensitivity. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.2 mg/mL
- 缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- 抗原
- Villin 1 (VIL1)
- 别名
- Villin (VIL1 产品)
- 别名
- D2S1471 antibody, VIL antibody, Vil antibody, vil1 antibody, MGC64339 antibody, VIL1 antibody, vil1l antibody, zgc:55779 antibody, villin 1 antibody, villin 1 S homeolog antibody, VIL1 antibody, Vil1 antibody, vil1.S antibody, vil1 antibody
- 背景
- Villin (VIL1) is an epithelial cell-specific Ca2+-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA), binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination. [UniProt]
- 基因ID
- 7429
- UniProt
- P09327
- 途径
- EGFR Signaling Pathway, Regulation of Actin Filament Polymerization
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