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ENO2/NSE 抗体 (AA 416-433)

ENO2 适用: 人 WB, IHC, IF, FACS 宿主: 小鼠 Monoclonal SPM347 unconjugated
产品编号 ABIN6252649
发货至: 中国
  • 抗原 See all ENO2/NSE (ENO2) 抗体
    ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
    抗原表位
    • 24
    • 19
    • 16
    • 16
    • 10
    • 9
    • 8
    • 7
    • 7
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    • 2
    • 2
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    • 2
    • 1
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    • 1
    • 1
    • 1
    • 1
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    • 1
    • 1
    • 1
    • 1
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    • 1
    AA 416-433
    适用
    • 184
    • 106
    • 93
    • 21
    • 6
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
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    • 2
    • 2
    • 1
    宿主
    • 134
    • 65
    • 2
    • 1
    • 1
    小鼠
    克隆类型
    • 137
    • 66
    单克隆
    标记
    • 95
    • 18
    • 17
    • 9
    • 6
    • 4
    • 4
    • 4
    • 4
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    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ENO2/NSE antibody is un-conjugated
    应用范围
    • 169
    • 69
    • 61
    • 52
    • 45
    • 40
    • 30
    • 27
    • 16
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    • 9
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    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
    纯化方法
    Protein G affinity chromatography
    免疫原
    Amino acids 416-433 of human Neuron Specific Enolase were used as the immunogen for this NSE antibody.
    克隆位点
    SPM347
    亚型
    IgG2b
    Top Product
    Discover our top product ENO2 Primary Antibody
  • 应用备注
    Western blot: 0.5-1 μg/mL

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    IF: 1-2 μg/mL

    IHC (FFPE): 0.1-0.2 μg/mL

    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.2 mg/mL
    缓冲液
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原
    ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
    别名
    Neuron Specific Enolase (ENO2 产品)
    别名
    ENO2 antibody, DKFZp459B1817 antibody, NSE antibody, AI837106 antibody, D6Ertd375e antibody, Eno-2 antibody, RNEN3 antibody, eno3 antibody, wu:fc09h05 antibody, zgc:92418 antibody, enolase 2 antibody, enolase 2 (gamma, neuronal) antibody, enolase 2, gamma neuronal antibody, enolase 2, gamma, neuronal antibody, ENO2 antibody, Eno2 antibody, eno2 antibody
    背景
    Recognizes a protein of about 50  kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
    基因ID
    2026
    UniProt
    P09104
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