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CA9 抗体

CA9 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal CBAD9-1 unconjugated
产品编号 ABIN6252593
发货至: 中国
  • 抗原 See all CA9 抗体
    CA9 (Carbonic Anhydrase IX (CA9))
    适用
    • 87
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    宿主
    • 73
    • 33
    小鼠
    克隆类型
    • 61
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    单克隆
    标记
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    This CA9 antibody is un-conjugated
    应用范围
    • 72
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    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    纯化方法
    Protein G affinity chromatography
    免疫原
    Recombinant human protein was used as the immunogen for the CAIX antibody.
    克隆位点
    CBAD9-1
    亚型
    IgG2b kappa
    Top Product
    Discover our top product CA9 Primary Antibody
  • 应用备注
    Western blot: 1-2 μg/mL



    Immunofluorescence: 1-2 μg/mL

    Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)

    Prediluted format: incubate for 30 min at RT (2)

    Titering of the CAIX antibody may be required for optimal performance.

    1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.2 mg/mL
    缓冲液
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原
    CA9 (Carbonic Anhydrase IX (CA9))
    别名
    CAIX (CA9 产品)
    别名
    CA9 antibody, CAIX antibody, Ca9 antibody, MN/CA9 antibody, MN antibody, carbonic anhydrase 9 antibody, CA9 antibody, CAH9 antibody, Car9 antibody
    背景
    Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. Carbonic anhydrase IX is a transmembrane protein and is one of only two tumor-associated carbonic anhydrase isoenzymes known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. [RefSeq]
    基因ID
    768
    UniProt
    Q16790
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