CA9 抗体
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- 抗原 See all CA9 抗体
- CA9 (Carbonic Anhydrase IX (CA9))
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适用
- 人, 马
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CA9 antibody is un-conjugated
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应用范围
- Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- The microsomal fraction of human renal cortical tissue homogenate was used as the immunogen for this Carbonic Anhydrase IX / CAIX antibody.
- 克隆位点
- 66-4-C2
- 亚型
- IgG2b kappa
- Top Product
- Discover our top product CA9 Primary Antibody
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anti-Carbonic Anhydrase IX (CA9) (AA 37-186) antibody
CA9 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 7C4A5 unconjugated
anti-Carbonic Anhydrase IX (CA9) (AA 59-414) antibodyCA9 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase IX (CA9) (AA 37-186) antibodyCA9 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 10F7A8 unconjugated
anti-Carbonic Anhydrase IX (CA9) antibodyCA9 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase IX (CA9) (AA 52-151) antibodyCA9 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase IX (CA9) (AA 113-143) antibodyCA9 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase IX (CA9) (AA 251-350) antibodyCA9 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Carbonic Anhydrase IX (CA9) antibodyCA9 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 9B5 unconjugated
anti-Carbonic Anhydrase IX (CA9) antibodyCA9 适用: 人 WB, IP 宿主: 小鼠 Monoclonal 12F10 unconjugated
anti-Carbonic Anhydrase IX (CA9) antibodyCA9 适用: 人, 马 WB, IHC, StM 宿主: 小鼠 Monoclonal CA9-781 unconjugated
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- 应用备注
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Flow Cytometry: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CAIX antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.2 mg/mL
- 缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- 抗原
- CA9 (Carbonic Anhydrase IX (CA9))
- 别名
- CAIX (CA9 产品)
- 别名
- CA9 antibody, CAIX antibody, Ca9 antibody, MN/CA9 antibody, MN antibody, carbonic anhydrase 9 antibody, CA9 antibody, CAH9 antibody, Car9 antibody
- 背景
- This antibody recognizes a glycoprotein of ~200 kDa, identified as renal cell carcinoma protein, carbonic anhydrase IX / CAIX, or gp200. Its epitope resides in the carbohydrate domain of the protein. The CAIX antibody shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, CAIX is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, it is expressed by 93 % of primary and 84 % of metastatic renal cell carcinomas. This CAIX antibody may be useful in the investigations of carcinomas of proximal nephrogenic differentiation, especially those showing tubular differentiation.
- 基因ID
- 768
- UniProt
- Q16790
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