HCG beta 抗体
-
- 抗原 See all HCG beta 抗体
- HCG beta
-
适用
- 人
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This HCG beta antibody is un-conjugated
-
应用范围
- Immunohistochemistry (IHC)
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Recombinant hCG beta protein was used as the immunogen for this hCG beta antibody.
- 克隆位点
- SPM529
- 亚型
- IgG1
- Top Product
- Discover our top product HCG beta Primary Antibody
-
-
- 应用备注
-
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)
The optimal dilution of the HCG-beta antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.2 mg/mL
- 缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
-
- 抗原
- HCG beta
- 别名
- HCG-beta (HCG beta 产品)
- 背景
- HCG is a glycoprotein which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. hCG mAb detects cells and tumors of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and adenocarcinoma of the lung demonstrate anti-hCG positivity in 90 % and 60 % of cases respectively. 20 % of lung squamous cell carcinomas are positive. hCG expression by non-trophoblastic tumors may indicate aggressive behavior.
- 基因ID
- 1082
- UniProt
- P01233
-