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MITF 抗体

MITF 适用: 人 IF, IHC (p) 宿主: 小鼠 Monoclonal MPAF3 unconjugated
产品编号 ABIN6252516
发货至: 中国
  • 抗原 See all MITF 抗体
    MITF (Microphthalmia-Associated Transcription Factor (MITF))
    适用
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    宿主
    • 90
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    小鼠
    克隆类型
    • 83
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    单克隆
    标记
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    This MITF antibody is un-conjugated
    应用范围
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    Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    特异性
    Does not react with Mouse or Rat
    纯化方法
    Protein G affinity chromatography
    免疫原
    Recombinant full-length human protein was used as the immunogen for the MITF antibody.
    克隆位点
    MPAF3
    亚型
    IgG1 kappa
    Top Product
    Discover our top product MITF Primary Antibody
  • 应用备注
    Immunofluorescence: 1-2 μg/mL

    Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)

    Prediluted format : incubate for 30 min at RT (2)

    Titering of the MITF antibody may be required for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.2 mg/mL
    缓冲液
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原
    MITF (Microphthalmia-Associated Transcription Factor (MITF))
    别名
    MITF (MITF 产品)
    别名
    MITF antibody, ws2a antibody, mitfa antibody, MITF-A antibody, LOC100313655 antibody, mitfb antibody, CMM8 antibody, MI antibody, WS2 antibody, WS2A antibody, bHLHe32 antibody, BCC2 antibody, Bhlhe32 antibody, Gsfbcc2 antibody, Vitiligo antibody, Wh antibody, bw antibody, mi antibody, vit antibody, MITF-H antibody, MITF-M antibody, CMI9 antibody, melanogenesis associated transcription factor antibody, microphthalmia-associated transcription factor antibody, microphthalmia-associated transcription factor S homeolog antibody, Microphthalmia antibody, MITF antibody, mitf antibody, LOC100313655 antibody, mitf.S antibody, LOC100534525 antibody, MICROP antibody, Mitf antibody
    背景
    MITF (microphthalmia transcription factor) is a transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. [UniProt]
    基因ID
    4286
    UniProt
    O75030
    途径
    Chromatin Binding
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