Myogenin 抗体
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- 抗原 See all Myogenin (MYOG) 抗体
- Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
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适用
- 人, 小鼠, 大鼠, Pig, Cat
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Myogenin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Human myogenin recombinant protein was used as the immunogen for this antibody.
- 克隆位点
- MGN185
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product MYOG Primary Antibody
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anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 4-219) antibody
MYOG 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 1-224) antibodyMYOG 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 2B7 unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) antibodyMYOG 适用: 人, 小鼠, 大鼠, Pig, Cow, 小鸡 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 1-224) antibodyMYOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E7 unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) antibodyMYOG 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 1-224) antibodyMYOG 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 1-224) antibodyMYOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E19 unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 1-1) antibodyMYOG 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 1-30) antibodyMYOG 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (Internal Region) antibodyMYOG 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Flow Cytometry: 0.5-1 μg/million cells
IF: 1-2 μg/mL
WB: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Myogenin antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.2 mg/mL
- 缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- 抗原
- Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
- 别名
- Myogenin (MYOG 产品)
- 别名
- cb553 antibody, zgc:100763 antibody, LOC446037 antibody, myf4 antibody, Xmyog antibody, myogenin antibody, LOC100136471 antibody, LOC100224062 antibody, LOC100303673 antibody, MYF4 antibody, bHLHc3 antibody, myo antibody, myf-4 antibody, myogenin antibody, myogenin (myogenic factor 4) antibody, MYOG antibody, myog antibody, LOC100303673 antibody, Myog antibody
- 背景
- Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80 % amino acid homology in their bHLH motifs. Myogenin antibody labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms' tumor.
- 基因ID
- 4656
- UniProt
- P15173
- 途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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