alpha Fetoprotein 抗体
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- 抗原 See all alpha Fetoprotein (AFP) 抗体
- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
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适用
- 人, Pig, 犬, 猴
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This alpha Fetoprotein antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- 特异性
- Does not react with cow, cat, mouse, rat.
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Alpha fetoprotein purified from serum of a hepatoma patient was used as the immunogen for this AFP antibody.
- 克隆位点
- C3
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product AFP Primary Antibody
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- 应用备注
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Flow Cytometry: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
IHC(FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the AFP antibody to be titered up or down for optimal performance.1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.2 mg/mL
- 缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Aliquot and Store at 2-8C. Avoid freez-thaw cycles.
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- 抗原
- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
- 别名
- Alpha Fetoprotein (AFP 产品)
- 别名
- FETA antibody, HPAFP antibody, alpha fetoprotein antibody, alpha-fetoprotein antibody, AFP antibody, Afp antibody
- 背景
- This antibody recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as Alpha fetoprotein (AFP). The AFP antibody is highly specific and shows no cross-reaction with other oncofetal antigens or serum albumin. Alpha fetoprotein is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. AFP antibody has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.
- 基因ID
- 174
- UniProt
- P02771
- 途径
- C21-Steroid Hormone Metabolic Process
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