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TTF1 抗体

TTF1 适用: 人, 大鼠, 小鼠 FACS, IHC (p), IF 宿主: 小鼠 Monoclonal NX2-1-690 unconjugated
产品编号 ABIN6252068
发货至: 中国
  • 抗原 See all TTF1 抗体
    TTF1 (Transcription Termination Factor, RNA Polymerase I (TTF1))
    适用
    人, 大鼠, 小鼠
    宿主
    • 48
    • 8
    小鼠
    克隆类型
    • 45
    • 11
    单克隆
    标记
    • 27
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This TTF1 antibody is un-conjugated
    应用范围
    • 33
    • 21
    • 15
    • 13
    • 10
    • 7
    • 6
    • 4
    • 3
    • 1
    • 1
    Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
    纯化方法
    Protein G affinity chromatography
    免疫原
    Recombinant protein was used as the immunogen for the TTF1 antibody.
    克隆位点
    NX2-1-690
    亚型
    IgG1 kappa
    Top Product
    Discover our top product TTF1 Primary Antibody
  • 应用备注
    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 0.5-1 μg/mL

    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

    Prediluted format: incubate for 30 min at RT (2)

    Optimal dilution of the TTF1 antibody should be determined by the researcher.

    1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.2 mg/mL
    缓冲液
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原
    TTF1 (Transcription Termination Factor, RNA Polymerase I (TTF1))
    别名
    TTF1 (TTF1 产品)
    别名
    TTF-1 antibody, TTF-I antibody, AV245725 antibody, RGD1565673 antibody, transcription termination factor 1 antibody, transcription termination factor, RNA polymerase I antibody, TTF1 antibody, Ttf1 antibody
    背景
    Recognizes a protein of 40  kDa, identified as Thyroid transcription factor-1. TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies.
    基因ID
    7080
    UniProt
    P43699
    途径
    Thyroid Hormone Synthesis, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour
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