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MYH11 抗体

MYH11 适用: 人, 大鼠, Cat, 小鸡, Cow, 犬, 豚鼠, Pig, 兔 IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal SPM201 unconjugated
产品编号 ABIN6251989
发货至: 中国
  • 抗原 See all MYH11 抗体
    MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))
    适用
    • 37
    • 20
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    人, 大鼠, Cat, 小鸡, Cow, 犬, 豚鼠, Pig, 兔
    宿主
    • 32
    • 8
    小鼠
    克隆类型
    • 20
    • 20
    单克隆
    标记
    • 23
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This MYH11 antibody is un-conjugated
    应用范围
    • 13
    • 12
    • 10
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    纯化方法
    Protein G affinity chromatography
    免疫原
    Human uterus extract was used as the immunogen for the SM-MHC antibody.
    克隆位点
    SPM201
    亚型
    IgG1 kappa
    Top Product
    Discover our top product MYH11 Primary Antibody
  • 应用备注
    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 0.5-1 μg/mL

    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

    Prediluted format: incubate for 30 min at RT (2)

    The stated application concentrations are suggested starting amounts. Optimal dilution of the SM-MHC antibody should be determined by the researcher.

    1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.2 mg/mL
    缓冲液
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原
    MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))
    别名
    SM-MHC (MYH11 产品)
    别名
    AV071570 antibody, SM1 antibody, SM2 antibody, smMHC antibody, AAT4 antibody, FAA4 antibody, SMHC antibody, SMMHC antibody, myosin antibody, myh11 antibody, myosin, heavy polypeptide 11, smooth muscle antibody, myosin heavy chain 11 antibody, myosin, heavy chain 11, smooth muscle antibody, myosin, heavy chain 11a, smooth muscle antibody, Myh11 antibody, MYH11 antibody, myh11a antibody
    背景
    Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors.
    基因ID
    4629
    UniProt
    P35749
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