Histone 3 抗体
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北京 101111
Quick Overview for Histone 3 抗体 (ABIN619545)
抗原
See all Histone 3 (H3) 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Anti-H3 | Histone H3 (rabbit antibody) (nuclear marker)
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交叉反应 （详细）
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Arabidopsis thaliana, Botrytis cinerea, Brassica oleracea, Camellia sinensis, Capsicum annuum, Cicer arietinum L., Chlamydomonas acidophila, Chlamydomonas reinhardtii, Cucumis sativus L cv Suyo, Cucurbita pepo L. var. cylindrica, Hordeum vulgare, human, Nicotiana benthamiana, Oryza sativa, Phalaenopsis equestris, Phaeodactylum tricornutum, Physcomitrium patens, Salicornia europaea, Stevia rebaudiana, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Vicia faba, Zea mays
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
预测反应
- Brachypodium distachyon,Brassica napus,Emiliania huxleyi,Fagopyrum tataricum Gaertn.,Glycine max,Nannochloropsis gaditana,Nicotiana tabacum,Malus domestica,Medicago sativa,Oryza sativa,Ostreococcus sp.,Pinus pinaster,Pisum sativum,Porphyridium purpureum,Saccharum sp.,Sorghum bicolor,Triticum aestivum,Vitis vinifera,Volvox sp.
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纯化方法
- Serum
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免疫原
- KLH-conjugated synthetic peptide derived from known H3 sequences
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anti-Histone H3 (H3) (3meLys4) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K4me) antibodyH3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K27ac) antibodyH3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K9ac) antibodyH3 适用: 人, 小鼠 WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K36me3) antibodyH3 适用: 人, 小鼠 WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 小鼠 Monoclonal MABI 0333 unconjugated
anti-Histone H3 (H3) (AA 71-136) antibodyH3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K27me) antibodyH3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K4me3) antibodyH3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3R2me2) antibodyH3 适用: 人 WB, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K36me2) antibodyH3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
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应用备注
- 1 : 100-1 : 500 (ICC), 2 μL of antibody/500 μL solution (ChIp-qPCR), 1: 500 (IF), 1 : 5000 (WB)
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说明
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Protocol for isolation of cytosolic and nuclear fractions can be found here.Specific fluorescence in ICC has been observed for interphase nuclei as well as around centromer region (where Ser10 of histone H3 is phosphorylated) in mitotic chromosomes.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- For reconstitution add 50 μL of sterile water
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缓冲液
- Serum
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "Protocol: Optimised methodology for isolation of nuclei from leaves of species in the Solanaceae and Rosaceae families." in: Plant methods, Vol. 9, Issue 1, pp. 31, (2013) (PubMed).
: "A maize cystatin suppresses host immunity by inhibiting apoplastic cysteine proteases." in: The Plant cell, Vol. 24, Issue 3, pp. 1285-300, (2012) (PubMed).
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- Histone 3 (H3) (Histone H3 (H3))
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别名
- H3
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背景
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Background: Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.
Additional information: Cellular [compartment marker] of nucleoplasm, loading control antibody for Chlamydomonas reinhardtii
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分子量
- 15 | 17 kDa
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基因ID
- 830164
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UniProt
- P59169, P59226, Q9FXI7
抗原
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