TYR 抗体 (Biotin)
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- 抗原 See all TYR 抗体
- TYR (Tyrosinase (TYR))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This TYR antibody is conjugated to Biotin
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应用范围
- Immunofluorescence (IF), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)
- 原理
- Mouse Monoclonal anti-Tyrosinase (OCA1/812), Biotin Conjugate
- 产品特性
- This antibody recognizes a cluster of proteins between 70-80 kDa, identified as tyrosinase. Occasionally a minor band at 55 kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway, the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 免疫原
- Recombinant human TYR protein
- 克隆位点
- OCA1-812
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product TYR Primary Antibody
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- 应用备注
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, or 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- ELISA For coating, order Ab without BSA
- Optimal dilution for a specific application should be determined by user
- 说明
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SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells or Melanoma.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- PBS/0.1 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- 抗原
- TYR (Tyrosinase (TYR))
- 别名
- Tyrosinase (TYR 产品)
- 别名
- sandy antibody, zgc:109705 antibody, TYR antibody, TYRO antibody, tyr antibody, LOC100136546 antibody, CMM8 antibody, OCA1A antibody, OCAIA antibody, SHEP3 antibody, albino antibody, c antibody, skc35 antibody, C antibody, tyrosinase antibody, TYRosinase antibody, tyrosinase S homeolog antibody, tyr antibody, tyr-5 antibody, tyr.S antibody, TYR antibody, Celal_3121 antibody, Halhy_4121 antibody, Mesop_2736 antibody, LOC100136546 antibody, Tyr antibody
- 背景
- ATN, CMM8, LB24-AB, Monophenol monooxygenase, OCA1, OCA1A, Oculocutaneous albinism IA, SHEP3, SK29-AB, Tumor rejection antigen AB, TYR
- 分子量
- 70-80 kDa
- 基因ID
- 7299, 503555
- UniProt
- P14679
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