Calprotectin 抗体
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- 抗原 See all Calprotectin (S100A8/A9) 抗体
- Calprotectin (S100A8/A9)
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Calprotectin antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 原理
- Mouse Monoclonal anti-Calprotectin (CPT/1028), Purified, with BSA
- 产品特性
- This antibody recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12 kDa alpha chain and a 14 kDa beta chain. Calprotectin comprises 60 % of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. Calprotectin has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.This MAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and is important for identifying macrophages in tissue sections. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 纯化方法
- Purified
- 免疫原
- Recombinant human Calprotectin protein
- 克隆位点
- CPT-1028
- 亚型
- IgM kappa
- Top Product
- Discover our top product S100A8/A9 Primary Antibody
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anti-Calprotectin (S100A8/A9) (AA 1-114) antibody
S100A8/A9 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calprotectin (S100A8/A9) (AA 1-113) antibodyS100A8/A9 适用: 小鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calprotectin (S100A8/A9) (AA 1-114) antibodyS100A8/A9 适用: 人 IHC, WB, IP, ICC 宿主: 小鼠 Monoclonal C6 unconjugated
anti-Calprotectin (S100A8/A9) antibodyS100A8/A9 适用: 人 IHC, ELISA, WB 宿主: 小鼠 Monoclonal 69-6-1 unconjugated
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- 应用备注
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Immunohistology formalin-fixed. Not suitable for frozen tissues. 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- 说明
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Tonsil, lymph node, or spleen
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 200 μg/mL
- 缓冲液
- PBS/0.05 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- 抗原
- Calprotectin (S100A8/A9)
- 别名
- Calprotectin (S100A8/A9 产品)
- 别名
- 60B8AG antibody, CAGB antibody, CFAG antibody, CGLB antibody, L1AG antibody, LIAG antibody, MAC387 antibody, MIF antibody, MRP14 antibody, NIF antibody, P14 antibody, Mrp14 antibody, MRP-14 antibody, p14 antibody, 60B8Ag antibody, AW546964 antibody, BEE22 antibody, Cagb antibody, GAGB antibody, L1Ag antibody, S100 calcium binding protein A9 antibody, S100 calcium binding protein A9 (calgranulin B) antibody, S100A9 antibody, S100a9 antibody
- 背景
- S100-A9, MRP14, calgranulin-B, calprotectin L1H subunit, leukocyte L1 complex heavy chain, migration inhibitory factor-related protein 14, S100 calcium-binding protein A9 (calgranulin B)
- 分子量
- 14 kDa
- 基因ID
- 6280, 112405
- UniProt
- P06702
- 途径
- S100 Proteins
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