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MITF 抗体 (CF®568)

MITF 适用: 人 IHC, IF, FACS 宿主: 小鼠 Monoclonal D5 CF®568
产品编号 ABIN6179974
发货至: 中国
  • 抗原 See all MITF 抗体
    MITF (Microphthalmia-Associated Transcription Factor (MITF))
    适用
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    宿主
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    小鼠
    克隆类型
    • 84
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    单克隆
    标记
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    This MITF antibody is conjugated to CF®568
    应用范围
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    Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
    原理
    Mouse Monoclonal anti-MiTF (Microphthalmia-Transcription Factor) (D5), CF568 Conjugate
    产品特性
    MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MITF, D5, recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    免疫原
    NH2 terminus fragment of human Mi protein
    克隆位点
    D5
    亚型
    IgG1 kappa
    Top Product
    Discover our top product MITF Primary Antibody
  • 应用备注
    Does not react with mouse or rat, others not tested
    • Immunohistology (formalin)
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user
    说明

    Jurkat, A-431, HeLa or 501 Mel human melanoma cells or Melanoma

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    100 μg/mL
    缓冲液
    PBS/0.1 % BSA/0.05 % azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Protect from light
  • 抗原
    MITF (Microphthalmia-Associated Transcription Factor (MITF))
    别名
    MiTF (Microphthalmia-Transcription Factor) (MITF 产品)
    别名
    MITF antibody, ws2a antibody, mitfa antibody, MITF-A antibody, LOC100313655 antibody, mitfb antibody, CMM8 antibody, MI antibody, WS2 antibody, WS2A antibody, bHLHe32 antibody, BCC2 antibody, Bhlhe32 antibody, Gsfbcc2 antibody, Vitiligo antibody, Wh antibody, bw antibody, mi antibody, vit antibody, MITF-H antibody, MITF-M antibody, CMI9 antibody, melanogenesis associated transcription factor antibody, microphthalmia-associated transcription factor antibody, microphthalmia-associated transcription factor S homeolog antibody, Microphthalmia antibody, MITF antibody, mitf antibody, LOC100313655 antibody, mitf.S antibody, LOC100534525 antibody, MICROP antibody, Mitf antibody
    背景
    BHLHE32, Class E basic helix-loop-helix protein 32 (bHLHe32), CMM8, Mi, Microphthalmia-associated transcription factor, MITF, WS2, WS2A
    分子量
    52-56 kDa (doublet)
    基因ID
    4286
    UniProt
    O75030
    途径
    Chromatin Binding
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