Keratin 5/8 抗体 (CF®568)
Quick Overview for Keratin 5/8 抗体 (CF®568) (ABIN6174464)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Monoclonal anti-Cytokeratin 5/8 (C-50), CF568 Conjugate
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产品特性
- This antibody recognizes keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Cytoskeletal preparation from HeLa cells
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亚型
- IgG1 kappa
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anti-Keratin 5/8 (KRT5/8) antibody
KRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, 兔 WB, FACS, IHC, IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal RCK102 unconjugated
anti-Keratin 5/8 (KRT5/8) antibodyKRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, Cow, 绵羊 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal C-50 unconjugated
anti-Keratin 5/8 (KRT5/8) antibodyKRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, Cow, 绵羊, 雪貂 WB, FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal C-50 unconjugated
anti-Keratin 5/8 (KRT5/8) antibodyKRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, 兔 WB, FACS, IHC, IHC (p), ICC, IHC (fro) 宿主: 小鼠 Monoclonal RCK102 unconjugated
anti-Keratin 5/8 (KRT5/8) antibodyKRT5/8 适用: 人, 小鼠, Pig, 大鼠, 犬, Hamster, Cow, 绵羊, 雪貂 WB, FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal SPM268 unconjugated
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应用备注
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Immunohistology formalin-fixed 0.5-1.0 μg/mL
- For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at RT
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 0.5-1.0 μg/mL
- Western blotting 0.5-1.0 μg/mL
- Does not react with chicken , or Xenopus
- Optimal dilution for a specific application should be determined by user
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说明
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HepG2 or A431 cells. Skin or Squamous cell carcinoma
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- Keratin 5/8 (KRT5/8)
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别名
- Cytokeratin 5/8
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分子量
- 58 kDa (CK5), 52.5 kDa (CK8)
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基因ID
- 3852, 3856
抗原
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