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Keratin 5/8 抗体 (CF®568)

This anti-Keratin 5/8 antibody is a 小鼠 单克隆 antibody detecting Keratin 5/8 in WB, FACS, IF 和 IHC (f). Suitable for 人, 小鼠, Pig, 大鼠, 犬, Hamster, Cow, 绵羊 和 雪貂.
产品编号 ABIN6174464
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Keratin 5/8 抗体 (CF®568) (ABIN6174464)

抗原

Keratin 5/8 (KRT5/8)

适用

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  • 16
  • 15
  • 13
  • 13
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人, 小鼠, Pig, 大鼠, 犬, Hamster, Cow, 绵羊, 雪貂

宿主

  • 23
小鼠

克隆类型

  • 23
单克隆

标记

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  • 4
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  • 1
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This Keratin 5/8 antibody is conjugated to CF®568

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

C-50
  • 原理

    Mouse Monoclonal anti-Cytokeratin 5/8 (C-50), CF568 Conjugate

    产品特性

    This antibody recognizes keratin 5 (58 kDa) and keratin 8 (52.5 kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Cytoskeletal preparation from HeLa cells

    亚型

    IgG1 kappa
  • 应用备注

    Immunohistology formalin-fixed 0.5-1.0 μg/mL
    • For staining of formalin-fixed tissues, digest sections with trypsin at 1 mg/mL PBS, 15 min at RT
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 0.5-1.0 μg/mL
    • Western blotting 0.5-1.0 μg/mL
    • Does not react with chicken , or Xenopus
    • Optimal dilution for a specific application should be determined by user

    说明

    HepG2 or A431 cells. Skin or Squamous cell carcinoma

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    Keratin 5/8 (KRT5/8)

    别名

    Cytokeratin 5/8

    分子量

    58 kDa (CK5), 52.5 kDa (CK8)

    基因ID

    3852, 3856
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