Myogenin 抗体 (AA 30-224) (CF®594)
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- 抗原 See all Myogenin (MYOG) 抗体
- Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
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抗原表位
- AA 30-224
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适用
- 人, 小鼠, 大鼠, Pig, Cat
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Myogenin antibody is conjugated to CF®594
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应用范围
- Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- Mouse Monoclonal anti-Myogenin (MGN185 + F5D), CF594 Conjugate
- 产品特性
- Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80 % amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms? tumor. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 免疫原
- Human myogenin recombinant protein (MGN185), Rat myogenin recombinant fragment containing amino acid 30-224 (F5D)
- 克隆位点
- MGN185 F5D
- 亚型
- IgG
- Top Product
- Discover our top product MYOG Primary Antibody
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anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 4-219) antibody
MYOG 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 1-224) antibodyMYOG 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 2B7 unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) antibodyMYOG 适用: 人, 小鼠, 大鼠, Pig, Cow, 小鸡 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 1-224) antibodyMYOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E7 unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) antibodyMYOG 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 1-224) antibodyMYOG 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 1-224) antibodyMYOG 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2E19 unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 1-1) antibodyMYOG 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (AA 1-30) antibodyMYOG 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Myogenin (Myogenic Factor 4) (MYOG) (Internal Region) antibodyMYOG 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistology (formalin)
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- 说明
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Rh-30 cells. Skeletal muscle or rhabdomyosarcoma.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- PBS/0.1 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Protect from light
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- 抗原
- Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))
- 别名
- Myogenin (MYOG 产品)
- 别名
- cb553 antibody, zgc:100763 antibody, LOC446037 antibody, myf4 antibody, Xmyog antibody, myogenin antibody, LOC100136471 antibody, LOC100224062 antibody, LOC100303673 antibody, MYF4 antibody, bHLHc3 antibody, myo antibody, myf-4 antibody, myogenin antibody, myogenin (myogenic factor 4) antibody, MYOG antibody, myog antibody, LOC100303673 antibody, Myog antibody
- 背景
- BHLHc3, cb553, Class C basic helix-loop-helix protein 3, Myf-4, MYF4, MYOG, Myogenic factor 4
- 分子量
- 34 kDa
- 基因ID
- 4656, 2830
- UniProt
- P15173
- 途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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