CD31 抗体
Quick Overview for CD31 抗体 (ABIN6169356)
抗原
See all CD31 (PECAM1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Monoclonal anti-CD31 / PECAM-1 (JC70/A + C31.3), Purified, with BSA
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产品特性
- CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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纯化方法
- Purified
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免疫原
- Human recombinant CD31 protein (C31.3) & Membrane preparation of a spleen from a patient with hairy cell leukemia (JC/70A)
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亚型
- IgG
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anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 601-680) antibody
PECAM1 适用: 人, 小鼠, 大鼠, 兔, Pig, 犬 FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (C-Term) antibodyPECAM1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IHC (p), IHC (f) 宿主: 山羊 Polyclonal unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 28-382) antibodyPECAM1 适用: 人 FACS, WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 681-738) antibodyPECAM1 适用: 人, 小鼠, 大鼠, 兔, Pig, Cow FACS, WB, ELISA, IHC (p), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 28-315) antibodyPECAM1 适用: 人 FACS, WB, IHC, IF, ELISA 宿主: 小鼠 Monoclonal 9E6E12 unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 29-738) antibodyPECAM1 适用: 人 WB, ELISA, IP, IHC (p) 宿主: 小鼠 Monoclonal 1D2-1A5 unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) antibodyPECAM1 适用: 人, 兔, Cynomolgus FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal JC-70A unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) antibodyPECAM1 适用: 人, 大鼠 FACS, WB, IHC, IF, StM 宿主: 小鼠 Monoclonal C31-3 unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) (AA 625-738) antibodyPECAM1 适用: 人 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal PECAM1-3527 unconjugated
anti-Platelet/endothelial Cell Adhesion Molecule (PECAM1) antibodyPECAM1 适用: 人, 大鼠, 兔, Cynomolgus FACS, WB, IHC, StM 宿主: 小鼠 Monoclonal C31-7 unconjugated
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应用备注
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Immunohistology formalin-fixed 1-2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
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说明
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Jurkat cells. Tonsil or Angiosarcoma.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 200 μg/mL
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缓冲液
- PBS/0.05 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
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别名
- CD31 / PECAM-1
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背景
- EndoCAM, PECA1, Platelet Endothelial Cell Adhesion Molecule 1, GPIIA',
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分子量
- ~100 kDa (endothelium) and ~130 kDa (platelets)
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基因ID
- 5175
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UniProt
- P16284
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途径
- Regulation of Actin Filament Polymerization
抗原
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