Desmocollin 2+3 (Extracellular Domain) 抗体
适用: 人, 小鼠, 大鼠
IF, WB, FACS, IHC
宿主: 小鼠
Monoclonal
7G6
unconjugated
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- 抗原
- Desmocollin 2+3
- 抗原表位
- Extracellular Domain
- 适用
- 人, 小鼠, 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC)
- 原理
- Mouse Monoclonal anti-Desmocollin-2/3 (7G6), Purified, with BSA
- 产品特性
- Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. The desmosome is subdivided into two regions. The plaque region lies adjacent to the plasma, and is believed to contain molecules that attach the intermediate filament cytoskeleton to the desmosome. The core region is composed of transmembrane glycoproteins that are thought to mediate cell-cell adhesion. Desmogleins and desmocollins are the main desmosomal transmembrane proteins. These desmosomal glycoproteins belong to the members of the cadherin family of adhesion molecules. Three different isoforms of both desmogleins and desmocollins have been identified, named as desmoglein 1-3 and desmocollins. Desmosomal cadherins showed differentiation-specific expression in the human epidermis, although the functional significance of this differential expression is not fully understood. Desmocollin-1 can be found in the upper layers. The expression of desmocollin-2 varies in the basal and suprabasal layers. And desmocollin-3 is expressed more evenly throughout the suprabasal layers. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 纯化方法
- Purified
- 免疫原
- Human desmocollin-2 extracellular domain (exact sequence is proprietary)
- 克隆位点
- 7G6
- 亚型
- IgG1 kappa
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anti-Desmocollin 2+3 (Extracellular Domain) antibody
适用: 人, 小鼠, 大鼠 IF, WB, FACS, IHC (p) 宿主: 小鼠 Monoclonal 7G6 unconjugated
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- 应用备注
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Immunohistology (formalin) 1-2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- 说明
-
HeLa cells. Skin
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 200 μg/mL
- 缓冲液
- PBS/0.05 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- 抗原
- Desmocollin 2+3
- 别名
- Desmocollin-2/3
- 背景
- ARVD11, Cadherin family member 2, CDHF2, Desmocollin-2, Desmocollin-3, Desmosomal glycoprotein II,III, Desmosomal glycoprotein II, Desmosomal glycoprotein III, DG2, DGII/III, DSC2, DSC3
- 分子量
- 110 kDa (DSC2), 100 kDa (DSC3)
- 基因ID
- 1824, 95612
- UniProt
- Q02487
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