Actin, Muscle Specific 抗体
适用: 人, 兔, 大鼠, 犬, 小鼠, Cat, 小鸡
FACS, IF, IHC (f)
宿主: 小鼠
Monoclonal
HHF35
unconjugated
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- 抗原 See all Actin, Muscle Specific products
- Actin, Muscle Specific
- 适用
- 人, 兔, 大鼠, 犬, 小鼠, Cat, 小鸡
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Actin, Muscle Specific antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 原理
- Mouse Monoclonal anti-Muscle Specific Actin (HHF35), Purified, BSA-free
- 产品特性
- This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 纯化方法
- Purified
- 免疫原
- SDS extract of human myocardium.
- 克隆位点
- HHF35
- 亚型
- IgG1 kappa
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anti-Actin, Muscle Specific antibody
适用: 人, 兔, 大鼠 FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal SPM160 unconjugated
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- 应用备注
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Immunohistology formalin-fixed 0.25-0.5 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- 说明
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Muscle or sarcoma
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS (no BSA, no azide)
- 储存液
- Azide free
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- 抗原
- Actin, Muscle Specific
- 别名
- Muscle Specific Actin (Actin, Muscle Specific 产品)
- 背景
- ACTA, ACTA1, ACTA2, ACTC1, Actin, ACTSA, Alpha-2 actin, alpha skeletal muscle, Alpha-actin-1, Cardiac muscle alpha actin-1, Skeletal muscle alpha actin-1
- 分子量
- Multiple
- 基因ID
- 58, 59, 70
- UniProt
- P62736
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