G-CSF 抗体 (Biotin)
Quick Overview for G-CSF 抗体 (Biotin) (ABIN6161614)
抗原
See all G-CSF (CSF3) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Monoclonal anti-Granulocyte Colony Stimulating Factor (G-CSF) (CSF3) (CSF3/900), Biotin Conjugate
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产品特性
- This MAb recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.G-CSF is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSF's, which are identical except for a three amino acid deletion in the amino-terminus of one form of the protein have been isolated from human cells. Murine and human G-CSF's share 73 % sequence identity at the amino acid level. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the < a href="https://biotium.com/wp-content/uploads/2013/07/CF-dye-selection-guide.pdf" target="_blank" rel="noopener">CF® Dye Selection Guide for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Human recombinant full-length CSF3 protein
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亚型
- IgG1
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anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 155-198) antibody
CSF3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 1-207) antibodyCSF3 适用: 人 ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 8G5F7 unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 1-207) antibodyCSF3 适用: 人 ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 7E4F7 unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 30-207) antibodyCSF3 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 1-207) antibodyCSF3 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) antibodyCSF3 适用: 人, Macaque IHC, StM 宿主: 小鼠 Monoclonal rCSF3-900 unconjugated Recombinant Antibody
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) antibodyCSF3 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 47-205) antibodyCSF3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) (AA 27-200) antibodyCSF3 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 3 (Granulocyte) (CSF3) antibodyCSF3 适用: 人, Macaque IHC, StM 宿主: 兔 Monoclonal CSF3-3166R unconjugated Recombinant Antibody
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应用备注
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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HL60 cells. Tonsil or lymph node
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))
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别名
- Granulocyte Colony Stimulating Factor (G-CSF)
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背景
- Pluripoietin, Filgrastim, Lenograstim, CSF3OS, G-CSF, chromosome 17 open reading frame 33 (C17orf33), colony stimulating factor 3 (granulocyte)
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分子量
- 19 kDa
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基因ID
- 1440, 2233
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UniProt
- P09919
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途径
- Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
抗原
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