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G-CSF 抗体 (CF®488A)

This anti-G-CSF antibody is a 小鼠 单克隆 antibody detecting G-CSF in IF, FACS 和 IHC (f). Suitable for 人 和 Macaque.
产品编号 ABIN6161607
发货至: 中国

Quick Overview for G-CSF 抗体 (CF®488A) (ABIN6161607)

抗原

See all G-CSF (CSF3) 抗体
G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

适用

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人, Macaque

宿主

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小鼠

克隆类型

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单克隆

标记

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This G-CSF antibody is conjugated to CF®488A

应用范围

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Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

CSF3-900
  • 原理

    Mouse Monoclonal anti-Granulocyte Colony Stimulating Factor (G-CSF) (CSF3) (CSF3/900), CF488A Conjugate

    产品特性

    This MAb recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.G-CSF is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSF's, which are identical except for a three amino acid deletion in the amino-terminus of one form of the protein have been isolated from human cells. Murine and human G-CSF's share 73 % sequence identity at the amino acid level. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the < a href="https://biotium.com/wp-content/uploads/2013/07/CF-dye-selection-guide.pdf" target="_blank" rel="noopener">CF® Dye Selection Guide for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Human recombinant full-length CSF3 protein

    亚型

    IgG1
  • 应用备注

    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Optimal dilution for a specific application should be determined by user

    说明

    HL60 cells. Tonsil or lymph node

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

    别名

    Granulocyte Colony Stimulating Factor (G-CSF)

    背景

    Pluripoietin, Filgrastim, Lenograstim, CSF3OS, G-CSF, chromosome 17 open reading frame 33 (C17orf33), colony stimulating factor 3 (granulocyte)

    分子量

    19 kDa

    基因ID

    1440, 2233

    UniProt

    P09919

    途径

    Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
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