UBE2F 抗体 (N-Term)
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- 抗原 See all UBE2F 抗体
- UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))
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抗原表位
- N-Term
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适用
- 人, 小鼠, 大鼠, Cow, 犬, Pig
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This UBE2F antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 序列
- SKLKRDDGLK GSRT-C
- 特异性
- Recogfnizes NEDD8 conjugating enzyme (N Terminus).
- 交叉反应 （详细）
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Species reactivity (expected):Human, Mouse, Canine (Dog), Pig, Cow.
Species reactivity (tested):Rat. - 纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
- 免疫原
- Peptide with sequence from the N Terminus of the protein sequence according to NP_542409.1. Genename: UBE2F
- Top Product
- Discover our top product UBE2F Primary Antibody
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anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (AA 1-185) antibody
UBE2F 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) antibodyUBE2F 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) antibodyUBE2F 适用: 人, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (C-Term) antibodyVerified UBE2F 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (N-Term) antibodyVerified UBE2F 适用: 大鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (C-Term) antibodyUBE2F 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 豚鼠, 猴, 兔, 斑马鱼, Bat, Hamster, 小鸡, 非洲爪蟾 WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (N-Term) antibodyUBE2F 适用: 人, 小鼠, 大鼠, Cow, 马, 豚鼠, 猴, Bat, Hamster WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (AA 1-185) antibodyUBE2F 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (N-Term) antibodyUBE2F 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 豚鼠, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Conjugating Enzyme E2F (UBE2F) (Middle Region) antibodyUBE2F 适用: 人, 小鼠, 大鼠, Cow, 犬, 马, 豚鼠, 兔, 斑马鱼, Saccharomyces cerevisiae WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))
- 别名
- NCE2 (UBE2F 产品)
- 别名
- NCE2 antibody, 2510010F15Rik antibody, AI851109 antibody, RGD1307608 antibody, zgc:77005 antibody, ubiquitin conjugating enzyme E2 F (putative) antibody, NEDD8-conjugating enzyme UBE2F antibody, ubiquitin-conjugating enzyme E2F (putative) antibody, ubiquitin conjugating enzyme E2F (putative) S homeolog antibody, UBE2F antibody, Tsp_01616 antibody, ube2f antibody, ube2f.S antibody, Ube2f antibody
- 背景
- Ubiquitin is covalently attached to target proteins by a multienzymatic system consisting of E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin-ligating) enzymes. NEDD8, a ubiquitin-like protein, is conjugated to proteins in a manner analogous to ubiquitinylation. beta-amyloid precursor protein-binding protein-1 (APPBP1) can bind to NEDD8 in rabbit reticulocyte lysates. However, since APPBP1 shows similarity to only the N-terminal domain of an E1 enzyme, it must interact with a protein showing similarity to the C-terminal region of E1s. By searching sequence databases, a cDNAs encoding UBA3 was identified as the human homolog of yeast Uba3. The predicted 442-amino acid UBA3 protein shares 43 % sequence identity with yeast Uba3. In vitro, UBA3 formed a complex with APPBP1 and a thioester linkage with NEDD8. APPBP1/UBA3 complex may function as an E1-like enzyme for the activation of NEDD8.Synonyms: NEDD8-conjugating enzyme 2, UBE2F, Ubiquitin-conjugating enzyme E2 F
- 基因ID
- 140739
- NCBI登录号
- NP_542409
- UniProt
- Q969M7
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