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CD43 抗体 (Biotin)

SPN 适用: 人 FACS, IF, IHC (f) 宿主: 小鼠 Monoclonal 84-3C1 Biotin
产品编号 ABIN6159372
发货至: 中国
  • 抗原 See all CD43 (SPN) 抗体
    CD43 (SPN) (Sialophorin (SPN))
    适用
    • 146
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    宿主
    • 95
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    小鼠
    克隆类型
    • 84
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    • 1
    单克隆
    标记
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    This CD43 antibody is conjugated to Biotin
    应用范围
    • 103
    • 69
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    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    原理
    Mouse Monoclonal anti-CD43 (84-3C1), Biotin Conjugate
    产品特性
    This antibody recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop III). 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    免疫原
    Stimulated human leukocytes
    克隆位点
    84-3C1
    亚型
    IgG1 kappa
    Top Product
    Discover our top product SPN Primary Antibody
  • 应用备注
    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Optimal dilution for a specific application should be determined by user
    说明

    Paracortex in a tonsil or a reactive lymph node

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    100 μg/mL
    缓冲液
    PBS/0.1 % BSA/0.05 % azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • 抗原
    CD43 (SPN) (Sialophorin (SPN))
    别名
    CD43 (SPN 产品)
    别名
    CD43 antibody, GALGP antibody, GPL115 antibody, LSN antibody, A630014B01Rik antibody, Cd43 antibody, Galgp antibody, Ly-48 antibody, Ly48 antibody, Lsn antibody, Lsn1 antibody, gpL115 antibody, sialophorin antibody, SPN antibody, Spn antibody
    背景
    Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, Sialophorin, SPN
    分子量
    95, 115, or 135 kDa
    基因ID
    6693, 632188
    UniProt
    P16150
    途径
    Regulation of Leukocyte Mediated Immunity
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