Melanoma Marker 抗体 (Biotin)
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- 抗原 See all Melanoma Marker products
- Melanoma Marker
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Melanoma Marker antibody is conjugated to Biotin
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应用范围
- Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt)
- 原理
- Mouse Monoclonal anti-Melanoma Marker (HMB45 + M2-7C10 + M2-9E3 + T311), Biotin Conjugate
- 产品特性
- This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 免疫原
- Recombinant hMART-1 protein (M2-7C10, M2-9E3), Recombinant tyrosinase protein (T311), Extract of pigmented melanoma metastases from lymph nodes (HMB45)
- 克隆位点
- HMB45 M2-7C10 M2-9E3 T311
- 亚型
- IgG1, IgG2a, IgG2b kappa
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anti-Melanoma Marker antibody
适用: 人 IHC (p) 宿主: 小鼠 Monoclonal HMB45 unconjugated
anti-Melanoma Marker antibody适用: 人 IHC, IF 宿主: 小鼠 Monoclonal KBA-62 unconjugated
anti-Melanoma Marker antibody适用: 人, 犬, 小鼠 IHC, IF 宿主: 小鼠 Monoclonal PNL2 unconjugated
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- 应用备注
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Immunohistology (Formalin-fixed) 0.5-1.0 μg/mL
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Optimal dilution for a specific application should be determined by user
- 说明
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SK-MEL-13 and SK-MEL-19 Melanoma cell lines, Melanomas
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- PBS/0.1 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- 抗原
- Melanoma Marker
- Abstract
- Melanoma Marker 产品
- 背景
- Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A, TYR, PMEL17
- 分子量
- 20-22 kDa (doublet) (MART), 70-80 kDa (Tyrosinase), 90-100 kDa (gp100)
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