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V5 Epitope Tag 抗体 (Biotin)

WB, ELISA, IHC (fro), IHC 宿主: 小鼠 Monoclonal Biotin
产品编号 ABIN602522
发货至: 中国
  • 抗原 See all V5 Epitope Tag products
    V5 Epitope Tag
    适用
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    宿主
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    小鼠
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    标记
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    This V5 Epitope Tag antibody is conjugated to Biotin
    应用范围
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    Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC)
    序列
    GKPIPNPLLG LDST
    特异性
    Recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is, gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined.
    纯化方法
    Protein G purified
    免疫原
    Paramyxovirus SV5, Simian-Virus 5.

    Type of Immunogen: Virus
    亚型
    IgG2a
  • 应用备注
    Approved: ELISA, IHC, IHC-Fr, WB (1:1000 - 1:5000)

    Usage: Western Blot:1:1000-1:5000. ELISA. Immunohistochemistry (frozen). The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    IgG conjugated to Biotin. Supplied as a liquid, 0.1 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Aliquot to Avoid repeated freezing and thawing.
    储存条件
    4 °C,-20 °C
    储存方法
    May be stored at 4°C for short-term only. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Aliquots are stable for at least 1 year.
  • 抗原
    V5 Epitope Tag
    别名
    V5 Tag (V5 Epitope Tag 产品)
    物质类
    Tag
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