SPARC 抗体
Quick Overview for SPARC 抗体 (ABIN602083)
抗原
See all SPARC 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Recognizes mouse Osteonectin. Displays no cross-reactivity with recombinant human Osteonectin.
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纯化方法
- Protein G purified
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免疫原
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Recombinant mouse Osteonectin.
Type of Immunogen: Recombinant protein -
亚型
- IgG2b
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anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 101-200) antibody
SPARC 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 1-303) antibodySPARC 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1B2 unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 224-251) antibodySPARC 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibodySPARC 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 284-303) antibodySPARC 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 224-251), (C-Term) antibodySPARC 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB19869 unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-303) antibodySPARC 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 18-302) antibodySPARC 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 284-303), (C-Term) antibodySPARC 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC) (AA 1-303) antibodySPARC 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Approved: ELISA (0.5 - 1 μg/mL), Flo (25 μg/mL), IHC, IHC-Fr (25 μg/mL)
Usage: Suitable for use in ELISA, Flow Cytometry and Immunohistochemistry. ELISA: 0.5-1 μg/mL). Immunohistochemistry (frozen): 25 μg/mL detects SPARC on mouse ovary tissue. Flow Cytometry: 25 μg/mL. For intracellular staining, cells must be fixed and permeabilized using 4 % paraformaldehyde and 0.1 % saponin. Dilute this antibody to 25 μg/mL and add 10 μLof this solution to 1-5x10^5 cells in a total reaction volume 200uL. Following a 30 minute incubation, cells should be washed with 0.1 % saponin prior to adding 10 mL of a 25 μg/mL stock solution of a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1 % saponin prior to flow cytometric analysis. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Sterile PBS
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS, pH 7.4, 5 % trehalose
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储存条件
- -20 °C
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储存方法
- Lyophilized powder may be stored at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C. Further dilutions can be made in buffer.
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