Histone 3 抗体 (H3K4me2)
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北京 101111
Quick Overview for Histone 3 抗体 (H3K4me2) (ABIN577702)
抗原
See all Histone 3 (H3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- H3K4me2
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特异性
- To confirm the specificity of the antisera a dot blot system was used with amounts of peptides from 10-250 pmoles. Unmodified, monomethylated, dimethylated and trimethylated peptides surrounding the Lysine 4 site on Histone H3 were spotted in lanes 1-4 respectively. Lanes 5-8 contained peptides starting at residue 6, with the sequence, TARKSTGGKAPRKQLAT, encompassing the residue lysine 9 on Histone H3 that is unmodified, monomethylated, dimethylated and trimethylated respectively. Lanes 9-12 contained peptides encompassing the residue lysine 27 on Histone H3 that is unmodified, monomethylated, dimethylated and trimethylated respectively.
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交叉反应 （详细）
- Peptide immunogen sequence is highly conserved. Expected to react with Histone H3 from yeast to mam- mals.
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产品特性
- Reacts specifically with modified dimethylated Lysine4 in Histone H3
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纯化方法
- Purified
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免疫原
- Lysine4 dimethylated peptide of Histone H3
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anti-Histone H3 (H3) (3meLys4) antibody
H3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K4me) antibodyH3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K27ac) antibodyH3 适用: 人, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K9ac) antibodyH3 适用: 人, 小鼠 WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K36me3) antibodyH3 适用: 人, 小鼠 WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 小鼠 Monoclonal MABI 0333 unconjugated
anti-Histone H3 (H3) (AA 71-136) antibodyH3 适用: 人, 小鼠, 大鼠 WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K27me) antibodyH3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K4me3) antibodyH3 适用: 人, 小鼠, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3R2me2) antibodyH3 适用: 人 WB, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
anti-Histone H3 (H3) (H3K36me2) antibodyH3 适用: 人 WB, IHC, IF, ChIP, IP, ChIP-seq 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blotting and ChIP. Other applications not yet tested.Suggested dilution, 1:500-1:2,000 AbX™ Anti DiMe Lys4 Histone H3 Rabbit pAb WesteRN BLOttINg
Acid treated HeLa (10 μg) extract was Antibody A006-100UL was used in ChIP with treated with a 1:500 dilution of antibody murine embryonic stem cells (lane 1) or A006-100UL prior to visualization. erythroleukemia cells (lane 2). Top Panel: PCR primers specific for ß-globulin were used to amplify a 210 base pair promoter following DNA isolation. Lower Panel: Input DNA control.
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说明
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Reacts specifically with modified dimethylated Lysine4 in Histone H3 Supplied as a PBS solution Applications include Western blotting and ChIP
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Phosphate Buffered Saline at pH 7.2 containing 0.01 % Tween 20 and 0.09 % Kathon preservative
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储存液
- Sodium azide
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注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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储存条件
- 4 °C
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储存方法
- Short Term: 4°C. Extended: Aliquot and freeze at -20°C
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- Histone 3 (H3) (Histone H3 (H3))
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别名
- Histone H3
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背景
- The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. Histones have been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination. Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development. A diverse set of histone lysine methyltransferases has been identified, all but one of which contain a SET domain originally identified in the Drosophila, Enhancer of zeste and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing. Methylation of these lysine residues induces the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules. The discovery in 2004 of the histone demethylase LSD1, followed by the Jumonji demethylases JMJD1, JMJD2 and JHDM1 has shown that methylation is a reversible epigenetic process controlling cellular events. FORM AbX™ Lys4 Dimethyl Lys4 Histone H3 Rabbit Antibody is produced as diluted rabbit serum.
抗原
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