Histone 3 抗体 (C-Term)
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Quick Overview for Histone 3 抗体 (C-Term) (ABIN577700)
抗原
See all Histone 3 (H3) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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交叉反应 (详细)
- Peptide immunogen sequence is highly conserved. Expected to react with Histone H3 from yeast to mammals.
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产品特性
- Reacts with modified Histone H3
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纯化方法
- Purified
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免疫原
- C-Terminal peptide of Histone H3 which should be non-modified.
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应用备注
- Western blotting and ImmunoprecipitationSuggested dilution, 1:500-1:1,500 ChIP: 50 μL AbX™ Anti Histone H3 Rabbit pAb WesteRN BlOttINg 5 μL of acid treated HeLa extracts or recombinant Histone H3 were treated with a 1:500 dilution of antibody A004-100UL prior to visualization. Lane 1: HeLa Extract Lane 2: HeLa cells treated with sodium butyrate Lane 3: Recombinant human Histone H3 (0.2 μg)
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说明
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Reacts with modified Histone H3 Supplied as a PBS solution Applications include Western blotting and ChIP
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Phosphate Buffered Saline at pH 7.2 containing 0.1 % Tween 20 and 0.09 % Kathon preservative
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储存液
- Sodium azide
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注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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储存条件
- 4 °C
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储存方法
- Short Term: 4°C. Extended: Aliquot and freeze at -20°C
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- Histone 3 (H3) (Histone H3 (H3))
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别名
- Histone H3
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背景
- Chromosomal surfaces are ornamented with a variety of posttranslational modifications of histones, which are required for the regulation of many of the DNA-templated processes. Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminus tail, H3 is involved with the 'beads on a string' structure of the nucleosome. Histone H3 is the most extensively post-translationally modified of the five histones. The term "Histone H3" alone is purposely ambigu- ous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the regulation of genes. For example, histone methylation either on their arginine and/ or lysine residues, yields a more diverse array of cellular functions.
抗原
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