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HIST1H4A 抗体 (acLys5, acLys8, acLys12)

Name: HIST1H4A 抗体 (acLys5, acLys8, acLys12)
SKU: ABIN5773871
Availability: OutOfStock

HIST1H4A 适用: 人, 小鼠 ELISA, WB, IF, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated

Western Blot analysis of (1) 25 ug whole cell extracts of Hela cells, (2) 15 ug histone extracts of Hela cells, (3) 1 ug of recombinant histone H2A, (4) 1 ug of recombinant histone H2B, (5) 1 ug of recombinant histone H3, (6) 1 ug of recombinant histone H4.

ChIP was performed on sheared chromatin from 100,000 K562 cells.

ELISA

ChIP assays were performed using human K562 cells.

Immunofluorescent staining of Hela cell line with antibody followed by (A) an anti-rabbit antibody conjugated to Alexa488, (B) antibody after incubation of the antibody following blocking peptides: H4K5,8,12 unmodified, (C) H4K5,8,12ac, (D) H2A.

Cross reactivity test using the Histone H4 (K5,8,12ac) antibody.

6 images

产品编号 ABIN5773871

发货至: 中国

Datasheet as PDF

抗原 See all HIST1H4A 抗体

HIST1H4A (Histone Cluster 1, H4a (HIST1H4A))
抗原表位
10

8

7

6

6

6

6

5

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
acLys5, acLys8, acLys12
适用
102

17

8

2

2

1

1

1
人, 小鼠
宿主
98

5
兔
Compatible Secondaries
克隆类型
87

16
多克隆
标记
94

3

2

2

1

1
This HIST1H4A antibody is un-conjugated
应用范围
80

60

34

32

29

23

18

15

11

10

4

3

1

1

1
ELISA, Western Blotting (WB), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq)

原理

Rabbit polyclonal antibody raised against synthetic peptide of Histone H4 (K5,8,12ac).

交叉反应

人, 小鼠

免疫原

A synthetic peptide (conjugated with KLH) corresponding to Histone H4, acetylated at lysines 5, 8, and 12.
anti-Histone Cluster 1, H4a (HIST1H4A) (3meLys20) antibody
HIST1H4A 适用: 人, 小鼠 ELISA, WB, IF, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (acLys5) antibody
HIST1H4A 适用: 人, 小鼠 ELISA, WB, IF, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (acLys12), (acLys16), (acLys5), (acLys8) antibody
HIST1H4A 适用: 人, 小鼠 ELISA, IF, ChIP, DB, ChIP-seq 宿主: 兔 Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (acLys8) antibody
HIST1H4A 适用: 人, 小鼠 ELISA, WB, IF, ChIP, DB 宿主: 兔 Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (meLys20) antibody
HIST1H4A 适用: 人 ELISA, WB, IHC, DB, FACS 宿主: 兔 Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (acLys31) antibody
HIST1H4A 适用: 人 ELISA, WB, IF, ChIP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (acLys16) antibody
HIST1H4A 适用: 人 ELISA, WB, IF, ChIP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Histone Cluster 1, H4a (HIST1H4A) (acLys16) antibody
HIST1H4A 适用: 人 ELISA, WB, IF, ICC, FACS 宿主: 兔 Monoclonal 2B8 unconjugated Recombinant Antibody

anti-Histone Cluster 1, H4a (HIST1H4A) (acLys56) antibody
HIST1H4A 适用: 人 ELISA, WB, IF, ICC 宿主: 兔 Monoclonal 2C9 unconjugated Recombinant Antibody

anti-Histone Cluster 1, H4a (HIST1H4A) (acLys12) antibody
HIST1H4A 适用: 人 ELISA, IF, ChIP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA (1:1000)
Western Blot (1:1000)
ChIP (0.5-1 μg/IP)
Dot Blot (1:20000)
Immunofluorescence (1:500)
The optimal working dilution should be determined by the end user.

限制

仅限研究用
状态

Liquid

缓冲液

In PBS (0.05 % sodium azide, 0.05 % proclin 300).

储存液

ProClin, Sodium azide

注意事项

This product contains ProClin and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

储存条件

-20 °C,-80 °C

储存方法

Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.
抗原

HIST1H4A (Histone Cluster 1, H4a (HIST1H4A))

别名

HIST1H4A (HIST1H4A 产品)

别名

Hist1h4a antibody, H4FA antibody, histone cluster 1, H3f antibody, histone cluster 1 H4 family member a antibody, histone cluster 1, H4a antibody, Hist1h3f antibody, HIST1H4A antibody, Hist1h4a antibody

背景

Full Gene Name: histone cluster 1, H4a
Synonyms: H4/a,H4FA

基因ID

8359