NSF 抗体
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- 抗原 See all NSF 抗体
- NSF (N-Ethylmaleimide-Sensitive Factor (NSF))
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适用
- 人, 大鼠, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NSF antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Antigen affinity purified
- 免疫原
- A recombinant human partial protein corresponding to amino acids N620-D744 was used as the immunogen for the NSF antibody.
- 亚型
- IgG
- Top Product
- Discover our top product NSF Primary Antibody
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anti-N-Ethylmaleimide-Sensitive Factor (NSF) (AA 590-744) antibody
NSF 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor (NSF) (AA 620-744) antibodyNSF 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor (NSF) (AA 1-250) antibodyNSF 适用: 人 WB, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor (NSF) antibodyNSF 适用: 大鼠, 小鼠 WB, ELISA, IHC, IP, ICC 宿主: 小鼠 Monoclonal 83-11 unconjugated
anti-N-Ethylmaleimide-Sensitive Factor (NSF) (AA 668-696), (C-Term) antibodyNSF 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB41933 unconjugated
anti-N-Ethylmaleimide-Sensitive Factor (NSF) antibodyNSF 适用: 人, 大鼠, 小鼠, 斑马鱼 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor (NSF) (Internal Region) antibodyNSF 适用: 人, 大鼠, 小鼠 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor (NSF) (C-Term) antibodyNSF 适用: 人, 大鼠, 小鼠, 斑马鱼, Cow, Pig, 兔, 犬, 马, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor (NSF) (AA 733-744) antibodyNSF 适用: 人, 大鼠, 小鼠, Hamster WB, ELISA, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-N-Ethylmaleimide-Sensitive Factor (NSF) (Internal Region) antibodyNSF 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal dilution of the NSF antibody should be determined by the researcher.\. Western Blot: 0.5-1 μg/mL,IHC (FFPE): 1-2 μg/mL,Direct ELISA: 0.1-0.5 μg/mL
- 限制
- 仅限研究用
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- 缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
- 储存条件
- -20 °C
- 储存方法
- After reconstitution, the NSF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- 抗原
- NSF (N-Ethylmaleimide-Sensitive Factor (NSF))
- 别名
- NSF / Vesicle-fusing ATPase (NSF 产品)
- 别名
- SKD2 antibody, AI316878 antibody, AU020090 antibody, AU067812 antibody, NSF antibody, 18.m06598 antibody, nsf antibody, si:bz18k17.1 antibody, wu:fj33g11 antibody, N-ethylmaleimide sensitive factor antibody, T1J1.4 antibody, T1J1_4 antibody, NEM-sensitive fusion protein antibody, N-ethylmaleimide sensitive factor, vesicle fusing ATPase antibody, N-ethylmaleimide sensitive fusion protein antibody, N-ethylmaleimide-sensitive factor antibody, N-ethylmaleimide sensitive factor antibody, N-ethylmaleimide-sensitive fusion protein, putative antibody, N-ethylmaleimide sensitive factor L homeolog antibody, vesicle-fusing ATPase antibody, N-ethylmaleimide-sensitive factor a antibody, AAA-type ATPase family protein antibody, NSF antibody, Nsf antibody, nsf antibody, BBOV_II007200 antibody, TGME49_318510 antibody, nsf.L antibody, LOC100180685 antibody, LOC9310886 antibody, nsfa antibody
- 背景
- N-ethylmaleimide-sensitive factor, also known as NSF, is an enzyme which in humans is encoded by the NSF gene. NSF is a homohexameric AAA ATPase involved in membrane fusion. NSF is ubiquitously found in the cytoplasm of eukaryotic cells. It is a central component of the cellular machinery in the transfer of membrane vesicles from one membrane compartment to another. During this process, SNARE proteins on two joining membranes (usually a vesicle and a target membrane such as the plasma membrane) form a tight complex. This aids fusion of the vesicle with the target membrane. It has been proposed that the role of NSF is to undo these SNARE complexes once membrane fusion has occurred, using the hydrolysis of ATP as an energy source, allowing the dissociated SNAREs to be recycled for reuse in further rounds of membrane fusion. This proposal remains controversial, however. Recent work indicates that the ATPase function of NSF does not function in recycling of vesicles but rather functions in the act of fusing vesicles with the plasma membrane.
- UniProt
- P46459
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