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- 抗原 See all TNF alpha 抗体
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
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适用
- 人
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宿主
- 兔
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克隆类型
- 单克隆
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标记
- This TNF alpha antibody is un-conjugated
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应用范围
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Purified
- 纯度
- Protein A affinity chromatography
- 免疫原
- Recombinant human protein was used as the immunogen for the recombinant TNFa antibody.
- 克隆位点
- TNF-1500R
- 亚型
- IgG kappa
- Top Product
- Discover our top product TNF alpha Primary Antibody
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anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody
TNF alpha 适用: 人, 小鼠, 大鼠, 兔, 马 WB, ELISA, IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) antibodyTNF alpha 适用: 人, 大鼠 IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal TNFA-1172 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) antibodyTNF alpha 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) antibodyTNF alpha 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal M1-C4 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) antibody (FITC)TNF alpha 适用: 人, Pig, Non-Human Primate ICFC 宿主: 小鼠 Monoclonal MAb11 FITC
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-227) antibodyTNF alpha 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 86-150) antibodyTNF alpha 适用: 人, 小鼠, 大鼠, Pig, 兔 WB, ELISA, IHC (p), IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) antibodyTNF alpha 适用: 人, 小鼠 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal 1F6 unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibodyTNF alpha 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibodyTNF alpha 适用: 小鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated
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- 应用备注
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Optimal dilution of the recombinant TNFa antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 4-8 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1) - 限制
- 仅限研究用
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- 缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the recombinant TNFa antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- 抗原
- TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
- 别名
- TNF alpha (TNF alpha 产品)
- 背景
- This mAb recognizes human 17-26 kDa protein, which is identified as cytokine TNF-alpha (Tumor Necrosis Factor-alpha). TNF-alpha can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
- 途径
- NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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