IL1A 抗体
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- 抗原 See all IL1A 抗体
- IL1A (Interleukin 1 alpha (IL1A))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This IL1A antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)
- Supplier Product No.
- 209-401-302-0100
- Supplier
- Rockland
- 原理
- IL-1 Alpha Antibody
- 交叉反应 (详细)
- This antibody is primarily directed against the 17,000 MW human IL-1a and is useful in determining its presence in various assays. In general, this antibody also detects primate IL-1a in the same formats using similar dilutions.
- 产品特性
- Synonyms: Interleukin 1 alpha precursor antibody, rabbit anti-Interleukin1 alpha antibody, rabbit anti-IL-1 Alpha Antibody, Pre-interleukin 1 alpha antibody
- 过滤
- Sterile filtered
- 免疫原
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Immunogen: The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human IL-1a produced in E.coli. The MW of the recombinant IL-1a was 17,000. This is the cleavage site generated by the IL-1b converting enzyme (ICE, capase-1).
Immunogen Type: Recombinant Protein
- 亚型
- IgG
- Top Product
- Discover our top product IL1A Primary Antibody
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- 应用备注
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Flow Cytometry Dilution: User Optimized
Immunohistochemistry Dilution: 1:100 to 1:200
Application Note: This IgG fraction antibody of anti-Human IL-1a has been tested for use in immunoblotting. This antibody is suitable for use in ELISA, neutralizations, immunohistochemistry, and flow cytometry. It recognizes the 17,000 MW mature IL-1a. Reactivity in other immunoassays is unknown.
Neutralization Dilution: User Optimized
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:1,000 - 1:5,000
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IL1A (Interleukin 1 alpha (IL1A))
- 别名
- IL1A (IL1A 产品)
- 背景
- Background: Interleukin 1 alpha is produced by activated macrophages. Anti-IL-1 alpha antibody is ideal for investigators involved in Immunology research.
- 基因ID
- 3552
- UniProt
- P01583
- 途径
- NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
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