NF-kB p65 抗体 (acLys310)
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北京 101111
Quick Overview for NF-kB p65 抗体 (acLys310) (ABIN5706804)
抗原
See all NF-kB p65 (NFkBP65) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- acLys310
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Supplier Product No.
- 600-401-j88
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Supplier
- Rockland
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原理
- NFkB [ac Lys 310] Antibody
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交叉反应 (详细)
- A BLAST analysis was used to suggest cross-reactivity with mouse and human based on 100 % sequence homology.
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产品特性
- Synonyms: rabbit anti-NFKB Ac Lys310 antibody, rabbit anti-NfKB K310ac antibody, NFKB, NFkß, NF-kB, NF-kappaB, NFkappaB, Transcription factor p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, RELA, NFKB3
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纯化方法
- Anti-NFkB [ac Lys 310] Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.
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过滤
- Sterile filtered
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免疫原
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Immunogen: Anti-NFkB K310ac affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic acetylated peptide surrounding Lysine 310 of human TF65 protein.
Immunogen Type: Conjugated Peptide
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亚型
- IgG
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应用备注
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-NFkB [ac Lys 310] Antibody has been tested in ELISA. Anti-NFkB K310ac is useful for in Immunohistochemistry and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~65 kDa corresponding to the appropriate cell lysate or extract.
Western Blot Dilution: 1 μg/mL
ELISA Dilution: 1:50,000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.05 mg/mL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol with 1 mg/mL Bovine Serum Albumin (BSA)
Preservative: None -
储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
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别名
- RELA
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背景
- Background: NFkB is a pleiotropic transcription factor present in most cell types and is the endpoint of a series of signal transduction events related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorgenesis and apoptosis. NFkB is contained in the cytoplasm inactively until it is released by a phosphorylated I-kappa-B, allowing the NFkB to translocate to the nucleus. This antibody is ideal for investigators interested in Metabolism, Cancer, Cardiovascular, Microbiology, Epigenetics and Nuclear Signaling, Immunology, Neuroscience, or Cell Biology research.
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UniProt
- Q04206
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途径
- NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins
抗原
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