TIAM1 抗体 (C-Term, Catalytic Domain)
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- 抗原 See all TIAM1 抗体
- TIAM1 (T-Cell Lymphoma Invasion and Metastasis 1 (TIAM1))
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抗原表位
- AA 904-1511, C-Term, Catalytic Domain
- 适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This TIAM1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Human Tiam1 (catalytic domain/ C-terminal domain) recognizes the C-terminal amino acids 904-1511 of Tiam 1 containing the DH-PH regions.
- 免疫原
- Human Tiam1 (catalytic domain/ C-terminal domain) is an affinity purified rabbit polyclonal antiserum derived by injection of rabbits with a GST fusion protein with the C-terminal amino acids 904-1511 of Tiam 1. The antiserum was purified by affinity chromatography using the fusion protein coupled to glutathione agarose beads.
- Top Product
- Discover our top product TIAM1 Primary Antibody
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anti-T-Cell Lymphoma Invasion and Metastasis 1 (TIAM1) (AA 1392-1591) antibody
TIAM1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-T-Cell Lymphoma Invasion and Metastasis 1 (TIAM1) (AA 52-224) antibodyTIAM1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-T-Cell Lymphoma Invasion and Metastasis 1 (TIAM1) (AA 1478-1506), (C-Term) antibodyTIAM1 适用: 人 WB 宿主: 兔 Polyclonal RB41848 unconjugated
anti-T-Cell Lymphoma Invasion and Metastasis 1 (TIAM1) (C-Term) antibodyVerified TIAM1 适用: 人 ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-T-Cell Lymphoma Invasion and Metastasis 1 (TIAM1) (AA 1478-1506) antibodyTIAM1 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-T-Cell Lymphoma Invasion and Metastasis 1 (TIAM1) (AA 1261-1397) antibodyTIAM1 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Human Tiam-1 (catalytic domain/ C-terminal domain) is suitable for immunoblotting, immunocytochemistry, and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.
- 限制
- 仅限研究用
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- 缓冲液
- Each vial contains 100 μL affinity purified rabbit antiserum containing 0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C, or in small aliquots at -20°C.
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- 抗原
- TIAM1 (T-Cell Lymphoma Invasion and Metastasis 1 (TIAM1))
- 别名
- Tiam 1 (TIAM1 产品)
- 别名
- TIAM1 antibody, tiam1 antibody, AI847750 antibody, D16Ium10 antibody, D16Ium10e antibody, T-cell lymphoma invasion and metastasis 1 antibody, T-cell lymphoma invasion and metastasis 1a antibody, T-cell lymphoma invasion and metastasis antibody, T cell lymphoma invasion and metastasis 1 antibody, TIAM1 antibody, tiam1a antibody, tiam1 antibody, Tiam1 antibody
- 背景
- Directional cell migration is essential for various physiological processes such as embryonic development, angiogenesis, wound healing, and tumor invasion. In response to extracellular and cell adhesion signals, cells acquire a polarized morphology with a leading edge at their front and a trailing tail at the rear. This front-rear polarity is established along the directional axis, with signaling molecules, adhesions, and the cytoskeleton distributed asymmetrically. The signaling molecules that control polarity include the Rho family GTPases, including Rac1. The Rac exchange factor Tiam1 participates in polarized cell migration. Tiam1 binds to integrins through talin and regulates Rac1 activity and adhesion turnover for polarized migration. Tiam 1 contains a Dbl homology (DH) or RhoGEF domain which consists of an ~ 150 amino acid region that induces Rho family GTPases to displace GDP. This effectively activates the Rho GTPase by allowing GTP binding, which is in excess over GDP in the cell. The DH domain is invariably preceded by a pleckstrin homology (PH) domain. While not absolutely required for catalysis of nucleotide exchange, the PH domain appears to greatly increase catalytic efficiency in many cases. Tiam1 (T-cell lymphoma invasion and metastasis inducing protein 1) was originally identified as an invasion-inducing gene. Thereafter several studies supported the suggestion that the Tiam1-Rac signaling pathway may be involved in the invasion and metastasis of tumor cells.
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