ELISA: (direct and indirect, 1:5,000-1:10,000). Western Blot: (1:5,000-1:10,000 using ECL, suggested blocking and dilution buffer is TBST with 0.05 % Tween 20 with 5 % skim milk, incubate 1 hour at room temperature). Optimal conditions must be determined individually for each application.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
Liquid. 0.2um-filtered solution in PBS, pH 7.4. Contains no preservatives.
储存液
Without preservative
储存条件
-20 °C
储存方法
Stable for at least 6 months after receipt when stored at -20°C.
ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.